Recombinant Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3138Y] to Aldolase B + Aldolase C
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Aldolase B + Aldolase C antibody [EPR3138Y]
See all Aldolase B+Aldolase C primary antibodies -
Description
Rabbit monoclonal [EPR3138Y] to Aldolase B + Aldolase C -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Aldolase B aa 250-350. The exact sequence is proprietary.
Database link: P05062 -
Positive control
- IHC: Human, mouse and rat kidney tissues. WB: Human, mouse and rat kidney lysate. His-tagged human Aldolase B and C full-length recombinant protein.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3138Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000. Predicted molecular weight: 39 kDa.
For unpurified use at 1/500 - 1/1000. |
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 39 kDa. For unpurified use at 1/500 - 1/1000. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Images
-
All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/200 dilution
Lane 1 : His-tagged human Aldolase A full-length recombinant protein
Lane 2 : His-tagged human Aldolase B full-length recombinant protein
Lane 3 : His-tagged human Aldolase C full-length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
-
All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/1000 dilution (Purified)
Lane 1 : Human kidney lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking Buffer and concentration: 5% NFDM/TBST
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab75751 has been referenced in 4 publications.
- Fahrner M et al. Proteome biology of primary colorectal carcinoma and corresponding liver metastases. Neoplasia 23:1240-1251 (2021). PubMed: 34768110
- Krotenberg Garcia A et al. Generation of mixed murine organoids to model cellular interactions. STAR Protoc 2:100997 (2021). PubMed: 34917977
- Wang C et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . PubMed: 24983750
- Tun HW et al. Pathway signature and cellular differentiation in clear cell renal cell carcinoma. PLoS One 5:e10696 (2010). IHC-P ; Human . PubMed: 20502531