BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
15 hour(s) and 0 minute(s) · Temperature: 4°C
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, Ale Host species: Goat Clonality: Polyclonal Conjugation: Alexa Fluor® 488
Dot-blot analysis with ab51253 shows it's specificity to phosphorylated alpha-synuclein it recognises recombinant phosphorylated and aggregated alpha-synuclein from transgenic mouse brain (TgM83).
ab51253 recognises pathological alpha-synuclein from human synucleinopathy brains, Parkinson's disease and multiple system atrophy.
ab51253 also detects insoluble alpha-synuclein aggregates within HEK 293T cells expressing human mutant A53T alpha-synuclein seeded with recombinant filaments of A53T alpha-synuclein. In unseeded HEk 293T cells expressing human mutant A53T, no phosphorylated and aggregated alpha-synuclein is detected.
Submitted Mar 01 2018
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