Recombinant Anti-Amphiphysin antibody [EP2060Y] (ab52646)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2060Y] to Amphiphysin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Amphiphysin antibody [EP2060Y]
See all Amphiphysin primary antibodies -
Description
Rabbit monoclonal [EP2060Y] to Amphiphysin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain lysate. IHC-P: Human brain tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2060Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. -
Tissue specificity
Neurons, certain endocrine cell types and spermatocytes. -
Sequence similarities
Contains 1 BAR domain.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 273 Human
- Entrez Gene: 218038 Mouse
- Entrez Gene: 60668 Rat
- Omim: 600418 Human
- SwissProt: P49418 Human
- SwissProt: Q7TQF7 Mouse
- SwissProt: O08838 Rat
- Unigene: 592182 Human
see all -
Alternative names
- Amp antibody
- AMPH 1 antibody
- AMPH antibody
see all
Images
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Anti-Amphiphysin antibody [EP2060Y] (ab52646) at 1/100000 dilution + Human brain lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 76 kDa
Observed band size: 128 kDa why is the actual band size different from the predicted? -
Ab52646 (1/100) staining human Amphiphysin in human brain tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab52646 has been referenced in 3 publications.
- Buser DP et al. Quantitative proteomics reveals reduction of endocytic machinery components in gliomas. EBioMedicine 46:32-41 (2019). PubMed: 31331834
- Bosch PJ et al. Proteomics Analysis of Dorsal Striatum Reveals Changes in Synaptosomal Proteins following Methamphetamine Self-Administration in Rats. PLoS One 10:e0139829 (2015). WB . PubMed: 26484527
- Kumar GS et al. Quantitative proteomics for identifying biomarkers for tuberculous meningitis. Clin Proteomics 9:12 (2012). IHC . PubMed: 23198679