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    amylase-assay-kit-colorimetric-ab102523.pdf

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Amylase Assay Kit (Colorimetric) (ab102523)

  • Datasheet
  • Protocol Booklet
Reviews (1)Q&A (14)References (26)

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Functional Studies - Amylase Assay Kit (ab102523)
  • Functional Studies - Amylase Assay Kit (ab102523)
  • Functional Studies - Amylase Assay Kit (ab102523)
  • Functional Studies - Amylase Assay Kit (ab102523)
  • Functional Studies - Amylase Assay Kit (ab102523)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 40 min
  • Sample type: Cell culture media, Cell culture supernatant, Serum, Tissue Extracts, Urine
  • Sensitivity: 0.2 mU/well

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Overview

  • Product name

    Amylase Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Urine, Serum, Tissue Extracts, Cell culture media
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    > 0.2 mU/well
  • Assay time

    0h 40m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Amylase Assay Kit (Colorimetric) ab102523 detects activity of α-amylase through a two-step reaction.


    In the amylase assay protocol, alpha-Amylase will cleave the substrate ethylidene-pNP-G7 to produce smaller fragments that are eventually modified by alpha-glucosidase. This causes the release of a chromophore that can then be measured at OD = 405 nm.


    The assay can detect α-amylase content as low as 0.2 mU.


    Amylase assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with a microplate reader every 2-3 min for 30-60 min


     

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K711 Amylase Activity Colorimetric Assay Kit. K711-100 is the same size as the 100 test size of ab102523.

    Amylases are enzymes that break starch down to sugar molecules. α-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for instance). α-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. 

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Amylase Assay Buffer NM 1 x 55ml
    Amylase Positive Control Red 1 vial
    Amylase Substrate Mix NM 1 x 5ml
    Nitrophenol standard Yellow 1 x 150µl
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Sugar Assays
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
  • Relevance

    Amylases are enzymes that break starch down to sugar molecules. Alpha-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker’s yeast for instance). Alpha-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring alpha-amylase activity is, therefore, very desirable.

Images

  • Functional Studies - Amylase Assay Kit (ab102523)
    Functional Studies - Amylase Assay Kit (ab102523)Image from Mondragon A et al., PLoS One. 2014;9(8):e104873. Fig 3.; doi: 10.1371/journal.pone.0104873. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Plasma amylase levels were measured (using ab102523) after 75 days treatment with saline or one of three drugs. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.

    There were no statistically significant changes in plasma amylase activity in mice that were administered with two of the drugs vs mice administered saline. However, administration of the third to animals on normal diet led to a 1.4-fold increase in amylase activity (p≤0.01, n = 3 per group) and a 1.3- fold increase in mice on a high fat diet (p≤0.01, n = 4 per group).

  • Functional Studies - Amylase Assay Kit (ab102523)
    Functional Studies - Amylase Assay Kit (ab102523)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Amylase Assay Kit (ab102523)
    Functional Studies - Amylase Assay Kit (ab102523)

    Amylase activity measured in biological fluids showing activity (mU) per mL of tested sample. Samples were diluted 2 fold.

  • Functional Studies - Amylase Assay Kit (ab102523)
    Functional Studies - Amylase Assay Kit (ab102523)

    Amylase activity measured in tissue lysates showing activity (mU) per mg of extracted protein.

    Protein concentration for samples varied from 16 mg/mL to 50 mg/mL. Samples were diluted 2 fold.

  • Functional Studies - Amylase Assay Kit (ab102523)
    Functional Studies - Amylase Assay Kit (ab102523)
    Sample Test

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (26)

Publishing research using ab102523? Please let us know so that we can cite the reference in this datasheet.

ab102523 has been referenced in 26 publications.

  • Zhao T  et al. Do proinflammatory cytokines play a role in clozapine-associated glycometabolism disorders? Psychopharmacology (Berl) 238:1979-1990 (2021). PubMed: 33774704
  • Wang J  et al. ROR?t inhibitor SR1001 alleviates acute pancreatitis by suppressing pancreatic IL-17-producing Th17 and ?d-T cells in mice with ceruletide-induced pancreatitis. Basic Clin Pharmacol Toxicol 129:357-368 (2021). PubMed: 34384137
  • Hernandez G  et al. Pancreatitis is an FGF21-deficient state that is corrected by replacement therapy. Sci Transl Med 12:N/A (2020). PubMed: 31915301
  • Itani K  et al. Varying starch to fat ratios in pelleted diets: I. Effects on nutrient digestibility and production performance in Eimeria-challenged broiler chickens. Br Poult Sci 61:703-709 (2020). PubMed: 32538137
  • Dai E  et al. Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathway. Nat Commun 11:6339 (2020). PubMed: 33311482
View all Publications for this product

Customer reviews and Q&As

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1-10 of 15 Abreviews or Q&A

Amylase activity kits with chicken intestinal samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
The Amylase Assay Kit (ab102523) used to measure amylase activity in chicken intestinal samples worked well.
However, due to multiple attempts in trying to figure out the right dilution (between 1:50 and 1:400) of fresh samples and due to high individual variation, we managed to test the kits on a very small number of birds (9) since we used all the amylase substrate bottle provided (5ml) and the nitrophenol standard when preparing standard curves. Better to use three dilutions first and test it on a small number of samples, then continue the analysis with the final chosen dilution. It is advisable to have 2 kits as the nitrophenol standard is not enough to prepare at least 2 standard curve. One standard curve consumes 90 microliter of the solution, and the amount provided is 150 microl- It would have been better if it was at least 180 or 200 microliter.

Abcam user community

Verified customer

Submitted Oct 30 2017

Question

Hi, Is this kit suitable for mouse serum amylase measurements?

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Abcam community

Verified customer

Asked on Feb 19 2014

Answer



I confirm this kit is suitable for measuring mouse serum amylase.

Read More

Ariana Veiga

Abcam Scientific Support

Answered on Feb 19 2014

Question

Dear,



Is the kit Amylase Assay Kit (ab102523) also usable for measurements with chicken plasma?



Thanks
Kind regards

Read More

Abcam community

Verified customer

Asked on Feb 25 2013

Answer

Thank you for contacting us and for your interest in our products.

I am pleased to confirm that the Amylase Assay Kit (ab102523) is suitable for the use with chicken plasma samples. Please do not hesitate to contact us in case you are experiencing any problems using chicken plasma with this kit.

I hope this is of help. Please do not hesitate to contact us again if you require further information.

Read More

Abcam Scientific Support

Answered on Feb 25 2013

Question

Are the two kits assay buffers the same? Can tissue extraction be performed using a phosphate buffer instead

Read More

Abcam community

Verified customer

Asked on Feb 20 2013

Answer


These 2 kit buffers have different components and pH. They cannot be used interchangeably and it is not recommended to perform the extraction with PBS.

Read More

Abcam Scientific Support

Answered on Feb 20 2013

Question

Inquiry: Hello, I have a question regarding two kits: ab102523 (amylase) and ab102531 (trypsin). I have invertebrate tissues to analyse. I would like to extract those tissues with a phosphate buffer. Will this buffer be suitable with both kits? If so what pH do you recommend (7.5)? If the phosphate is not ok, can I use one of the kit buffer (amylase or trypsin?)?

Read More

Abcam community

Verified customer

Asked on Feb 14 2013

Answer


The kit buffers would be ideal to extract your tissue. You can use the amylase assay buffer and the trypsin assay buffer.

Read More

Abcam Scientific Support

Answered on Feb 14 2013

Question

I have a few question about your product, Amylase Activity Assay Kit (ab102523).
1) Do you know whether this product react with any lipases?
2) Do you have a reaction formula about this product?
3) What did you use as a positive control?
4) If positive control is used with this kit, what is the activity value of it?"

Read More

Abcam community

Verified customer

Asked on Jan 24 2013

Answer

Thanks.


We are not aware of any lipase interference in this assay.
The reaction principle is mentioned on our datasheet for this product. The complete organic reaction is proprietary information. The same goes for the positive control identity and activity as well. Sorry!

Read More

Abcam Scientific Support

Answered on Jan 24 2013

Question

Thank you for your kind response. As a matter of fact I would like to know if the kit has any guideline or manual which directly pointed on saliva as a sample site and also I would be so grateful if you let me know about probable other previous similar researches that had used your kit on saliva.
Best Regards

Read More

Abcam community

Verified customer

Asked on Oct 01 2012

Answer

Thank you for contacting us.


The protocol can be found by clicking the following link;

https://www.abcam.com/ps/products/102/ab102523/documents/ab102523%20Amylase%20Assay%20Kit%20(Website).pdf

We do not have received any feedback from other researchers so I am sorry we will not be able to share any publication.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Abcam Scientific Support

Answered on Oct 01 2012

Question

Dear colleague
I'm XA conducting a research on occupational stress and willing to use your kit ab102523 for assaying alpha amylase on SALIVA, but in data you've provided the saliva has not been mentioned directly as the sample site. Please let me know if your kit can be used on measuring alpha amylase level in saliva.
Sincerely
Sent from my iPhone

Read More

Abcam community

Verified customer

Asked on Sep 25 2012

Answer

Thank you for your enquiry regarding ab102523.

I can confirm that this kit can be used for detecting Amylase in different sample types such as Cell culture supernatant, Urine, Serum, Plasma, Tissue Extracts, Cell culture media - as well as Other biological fluids. This means that it should work in saliva.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on Sep 25 2012

Question

ab102523
Can we use this kit on rat samples?

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Abcam community

Verified customer

Asked on Mar 29 2012

Answer

Thank you for your telephone enquiry this morning.

I can confirm that this kit has not been specifically tested on rat samples, and so regrettably there is no data to provide. However, theoretically it should work in rat and so we can guarantee it for use in this species.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Mar 29 2012

Question

Thank you for the offer. As I am going to use this kit for my plant protein which is not yet been understood and I do not know what type of antibody does it contain so, I would be grateful if you can provide me testing discount for the enzyme kit.

Read More

Abcam community

Verified customer

Asked on Feb 03 2012

Answer

Thank you for contacting us.

Unfortunately, at this time our testing discount program only applies to primary antibodies, proteins, and ELISA kits. I am sorry I could not be more helpful, please let me know if you need any further assistance.

Read More

Abcam Scientific Support

Answered on Feb 03 2012

1-10 of 15 Abreviews or Q&A

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