Anti-Amylin/DAP antibody (ab15125)
Key features and details
- Rabbit polyclonal to Amylin/DAP
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Amylin/DAP antibody
See all Amylin/DAP primary antibodies -
Description
Rabbit polyclonal to Amylin/DAP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Amylin/DAP aa 1 to the C-terminus (N terminal). The exact sequence is proprietary.
Database link: P10997 -
Positive control
- Pancreas.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/200 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min before commencing with IHC staining protocol. |
Notes |
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IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 1/200 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min before commencing with IHC staining protocol. |
Target
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Function
Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. -
Sequence similarities
Belongs to the calcitonin family. -
Domain
The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation. -
Post-translational
modificationsAmyloid fibrils are degraded by IDE. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3375 Human
- Omim: 147940 Human
- SwissProt: P10997 Human
- Unigene: 46835 Human
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Alternative names
- Amylin antibody
- DAP antibody
- Diabetes associated peptide antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab15125 has been referenced in 4 publications.
- Martinez-Valbuena I et al. Mixed pathologies in pancreatic ß cells from subjects with neurodegenerative diseases and their interaction with prion protein. Acta Neuropathol Commun 9:64 (2021). PubMed: 33832546
- Martinez-Valbuena I et al. Interaction of amyloidogenic proteins in pancreatic ß cells from subjects with synucleinopathies. Acta Neuropathol N/A:N/A (2018). IHC-P ; Human . PubMed: 29536165
- Ramzy A et al. Insulin-Deficient Mouse ß-Cells Do Not Fully Maturebut Can Be Remedied Through Insulin Replacementby Islet Transplantation. Endocrinology 159:83-102 (2018). PubMed: 29029025
- Suzuki H & Yamamoto T Amylin-like immunoreactivity in pancreatic X cells of the black-spotted frog Rana (Pelophylax) nigromaculata. Tissue Cell 46:535-9 (2014). IHC-Fr ; Frog . PubMed: 25458814