Annexin V/ANXA5 overexpression 293T lysate (whole cell) (ab76216)
Overview
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Product name
Annexin V/ANXA5 overexpression 293T lysate (whole cell)
See all Annexin V/ANXA5 lysates -
General notes
ab76216 is a 293T cell transfected lysate in which Human Annexin V/ANXA5 has been transiently over-expressed using a pCMV-Annexin V/ANXA5 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared, click here.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 2% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.79% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Research areas
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Background
Disease: Pregnancy loss, recurrent, 3 Domain: The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. A pair of annexin repeats may form one binding site for calcium and phospholipid. Function: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. PTM: S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. Similarity: Belongs to the annexin family. Contains 4 annexin repeats.
Associated products
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Corresponding Antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
Images
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ab76216 at 15μg/lane on an SDS-PAGE gel.
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All lanes : Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775) at 1/500 dilution
Lane 1 :Annexin V/ANXA5 overexpression 293T lysate (whole cell) (ab76216)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab76216 has not yet been referenced specifically in any publications.