Anti-Annexin-8/ANXA8 antibody (ab111693)
Key features and details
- Rabbit polyclonal to Annexin-8/ANXA8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin-8/ANXA8 antibody
See all Annexin-8/ANXA8 primary antibodies -
Description
Rabbit polyclonal to Annexin-8/ANXA8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit -
Immunogen
Recombinant fragment corresponding to Human Annexin-8/ANXA8 aa 1-188. (AAH04376)
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Positive control
- A549 and HeLa whole cell lysates; HeLa cells; U87 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. - Information by UniProt
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Database links
- Entrez Gene: 244 Human
- Entrez Gene: 653145 Human
- Entrez Gene: 11752 Mouse
- Entrez Gene: 306283 Rat
- Omim: 602396 Human
- SwissProt: P13928 Human
- SwissProt: O35640 Mouse
- SwissProt: Q4FZU6 Rat
see all -
Alternative names
- Annexin A8 antibody
- Annexin VIII antibody
- Annexin-8 antibody
see all
Images
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ab111693 at 1/200 dilution staining Annexin-8/ANXA8 in methanol-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
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Anti-Annexin-8/ANXA8 antibody (ab111693) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 37 kDa
10% SDS-PAGE. -
ab111693 at 1/500 dilution staining Annexin-8/ANXA8 in Formalin-fixed, paraffin-embedded U87 xenograft tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab111693 has been referenced in 1 publication.
- Ma F et al. Prognostic Value of ANXA8 in Gastric Carcinoma. J Cancer 11:3551-3558 (2020). PubMed: 32284751