Product nameAnnexin VIII overexpression 293T lysate (whole cell)
ab94176 is a 293T cell transfected lysate in which Human Annexin VIII has been transiently over-expressed using a pCMV-Annexin VIII plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituent: 100% 1x Sample Buffer
Concentration information loading...
PurityWhole Cell Lysate
BackgroundFunction: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.
Our Abpromise guarantee covers the use of ab94176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94176 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Annexin VIII antibody (ab73876) at 1/500 dilution
Lane 1 :
Annexin VIII overexpression 293T lysate (whole cell) (ab94176)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94176 has not yet been referenced specifically in any publications.