Anti-Apc1 (phospho S355) antibody (ab10923)
Key features and details
- Rabbit polyclonal to Apc1 (phospho S355)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Apc1 (phospho S355) antibody
See all Apc1 primary antibodies -
Description
Rabbit polyclonal to Apc1 (phospho S355) -
Host species
Rabbit -
Specificity
Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is expected to be phospho-specific for S355 of APC1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human Apc1 aa 350-450 (phospho S355) conjugated to keyhole limpet haemocyanin.
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General notes
This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This affinity purified antibody is directed against the phosphorylated form of human APC1 at the pS355 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
Primary antibody notes
This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
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Notes |
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WB
1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa). |
Target
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Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the APC1 family.
Contains 4 PC repeats. -
Post-translational
modificationsPhosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 535565 Cow
- Entrez Gene: 475750 Dog
- Entrez Gene: 64682 Human
- Entrez Gene: 17222 Mouse
- Omim: 608473 Human
- SwissProt: E1BAB6 Cow
- SwissProt: E2RLM2 Dog
- SwissProt: Q9H1A4 Human
see all -
Alternative names
- ANAPC 1 antibody
- Anapc1 antibody
- Anaphase promoting complex subunit 1 antibody
see all
Images
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All lanes : Anti-Apc1 (phospho S355) antibody (ab10923) at 1/500 dilution
Lane 1 : HeLa S3 whole cell lysate (asynchronous cells)
Lane 2 : HeLa S3 whole cell lysate (cells synchronized at G1/S boundary by treatment with thymidine)
Lane 3 : HeLa S3 whole cell lysate (cells synchronized at M phase by treatment with nocodazole)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG [H&L] at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 215 kDa
Observed band size: 215 kDa
Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab10923 has been referenced in 1 publication.
- Zhang Y et al. miR-29a suppresses IL-13-induced cell invasion by inhibiting YY1 in the AKT pathway in lung adenocarcinoma A549 cells. Oncol Rep 39:2613-2623 (2018). PubMed: 29620222