Overview

  • Product name
    Anti-Apolipoprotein A I antibody [EP1368Y]
    See all Apolipoprotein A I primary antibodies
  • Description
    Rabbit monoclonal [EP1368Y] to Apolipoprotein A I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Sandwich ELISA, IHC-FoFr, WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Apolipoprotein A I aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • Fetal liver lysate, human liver tissue and HEPG2 cell
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab52945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA 1/2000 - 1/4000. Can be paired for Sandwich ELISA with Goat polyclonal to Apolipoprotein A I (HRP) (ab20784). PubMed: 20421239
IHC-FoFr Use at an assay dependent concentration.
WB 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IP 1/60.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
    • Tissue specificity
      Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
    • Involvement in disease
      Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
      Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
      Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
      Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
    • Sequence similarities
      Belongs to the apolipoprotein A1/A4/E family.
    • Post-translational
      modifications
      Palmitoylated.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apo-AI antibody
      • ApoA I antibody
      • ApoA-I antibody
      • APOA1 antibody
      • APOA1_HUMAN antibody
      • Apolipoprotein A-I(1-242) antibody
      • Apolipoprotein A1 antibody
      • Apolipoprotein AI antibody
      • Brp14 antibody
      • Ltw1 antibody
      • Lvtw1 antibody
      • Sep1 antibody
      • Sep2 antibody
      see all

    Images

    • Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/20000 dilution + fetal liver lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa

    • ab52945 at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.
    • ab52945 at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.

    References

    This product has been referenced in:
    • Kim K  et al. C1QBP is upregulated in colon cancer and binds to apolipoprotein A-I. Exp Ther Med 13:2493-2500 (2017). Read more (PubMed: 28565870) »
    • Jattan J  et al. Using primary murine intestinal enteroids to study dietary TAG absorption, lipoprotein synthesis, and the role of apoC-III in the intestine. J Lipid Res 58:853-865 (2017). Read more (PubMed: 28159868) »
    See all 10 Publications for this product

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Small Intestine)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Small Intestine
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde

    Arnab Chattopadhyay

    Verified customer

    Submitted Oct 31 2018

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 1% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Feb 05 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain lysate)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brain lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Jan 28 2016

    Answer

    Thank you for your enquiry and interest in our products.

    Currently, the blocking peptide for this particular antibody is not available commercially. However, if you wish to check the specificity I would advise to run a no-primary (only secondary antibody) control, a positive control (fetal liver lysate, human liver tissue and HEPG2 cells) and a negative control alongside with the samples.

    You may find some useful information on how to find positive control at this site:

    https://www.abcam.com/index.html?pageconfig=technicalfaqs#6

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Answer

    Thank you for bringing this to our attention. I agree that your protocol appears correct and that the antibody may be at fault. We sometimes see an improvement in signal for some antibodies after switching the blocking buffer from 5% milk to BSA, but given that you see no signal at all with a dilution of 1/1000, trying a different antibody may be the better approach.

    The concentrations of most of our rabbit monoclonal antibodies are not listed on the datasheets because they are usually so low that customers become wary of buying them. These antibodies are unique in having very high affinity for their targets compared to mouse monoclonal antibodies and rabbit polyclonal antibodies. The lot that you have, YI041902C, has an IgG concentration of 0.112 mg/ml.

    The only two lots we currently have are the one you have and the one received by the customer you mentioned, who also failed to obtain a western blot signal. If you are not interested in trying a different antibody, I will send a refund. If you would like to try a different antibody, I will be happy to send that instead, such as ab7613, a goat polyclonal which was recently published here: J Biol Chem. 2012 June 1; 287(23): 19341–19345. The western blot is in figure 2, at the following link:

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3365970/figure/F2/

    Read More

    Answer

    Thank you for contacting us.

    I am sorry that the vial you received was not at the predicted concentration and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of xxxxxx.


    Please do not hesitate to contact us if you need anything further.

    Read More

    Answer

    Thank you for contacting us. Yes, the Apolipoprotein A I protein ab90760 is the correct target for the antibodies ab52945 and ab20918. However, please note that the only data we have for sELISA are : - ab52945 has been used in sELISA in pair with ab33470, - ab20918 has been used in sELISA in pair with ab64308. See product datasheet for more details : www.abcam.com/ab52945 www.abcam.com/ab33470 www.abcam.com/ab20918 www.abcam.com/ab64308 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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