Anti-Aquaporin 4 antibody [4/18] (ab9512)
Key features and details
- Mouse monoclonal [4/18] to Aquaporin 4
- Suitable for: IHC-P, IHC-Fr, IHC-FoFr, ELISA, WB
- Reacts with: Mouse, Rat, Rabbit, Human, Zebrafish
- Isotype: IgG3
Overview
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Product name
Anti-Aquaporin 4 antibody [4/18]
See all Aquaporin 4 primary antibodies -
Description
Mouse monoclonal [4/18] to Aquaporin 4 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, IHC-Fr, IHC-FoFr, ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Human, Zebrafish
Predicted to work with: Cow -
Immunogen
Synthetic peptide:
VIDIDRGDEKKGKDSSGE
, corresponding to amino acids 301-318 of Aquaporin 4 -
Epitope
Intracellular C-terminal AQP 4 epitope -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4/18 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (4) |
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
|
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
IHC-FoFr | (2) |
Use at an assay dependent concentration. PubMed: 22438975
|
ELISA |
Use at an assay dependent concentration. PubMed: 11595449
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. PubMed: 19831719
|
Notes |
---|
IHC-P
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 22438975 |
ELISA
Use at an assay dependent concentration. PubMed: 11595449 |
WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. PubMed: 19831719 |
Target
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Function
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. -
Tissue specificity
Brain - muscle >> heart, kidney, lung, and trachea. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Post-translational
modificationsPhosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 361 Human
- Entrez Gene: 11829 Mouse
- Entrez Gene: 25293 Rat
- Omim: 600308 Human
- SwissProt: O77750 Cow
- SwissProt: P55087 Human
- SwissProt: P55088 Mouse
- SwissProt: P47863 Rat
see all -
Alternative names
- AQP 4 antibody
- AQP-4 antibody
- AQP4 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (44)
ab9512 has been referenced in 44 publications.
- Sawicka E et al. The Influence of Interaction between Cadmium with 17β-Estradiol, 2-Methoxyestradiol and 16α-Hydroxyestrone on Viability and p-Glycoprotein in Ovarian Cancer Cell Line. Int J Mol Sci 23:N/A (2022). PubMed: 35269769
- Liu XL et al. Mesenchymal Stem Cells Improve Cognitive Impairment and Reduce Aβ Deposition via Promoting AQP4 Polarity and Relieving Neuroinflammation in Rats With Chronic Hypertension-Induced Cerebral Small-Vessel Disease. Front Aging Neurosci 14:883503 (2022). PubMed: 35663575
- Yang LY et al. Dental Pulp Stem Cell-Derived Conditioned Medium Alleviates Subarachnoid Hemorrhage-Induced Microcirculation Impairment by Promoting M2 Microglia Polarization and Reducing Astrocyte Swelling. Transl Stroke Res N/A:N/A (2022). PubMed: 36181630
- Jung E et al. Tumor cell plasticity, heterogeneity, and resistance in crucial microenvironmental niches in glioma. Nat Commun 12:1014 (2021). PubMed: 33579922
- Purohit D et al. Human Cord Blood Derived Unrestricted Somatic Stem Cells Restore Aquaporin Channel Expression, Reduce Inflammation and Inhibit the Development of Hydrocephalus After Experimentally Induced Perinatal Intraventricular Hemorrhage. Front Cell Neurosci 15:633185 (2021). PubMed: 33897371