Overview

  • Product name
    Arf1 Activation Assay Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Arf1 Activation Assay Kit (ab211170) uses GGA3 PBD Agarose beads to selectively isolate and pull-down the active form of Arf from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Arf is detected by western blot analysis using an anti-Arf1 antibody, which detects the human, mouse and rat protein.


    Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.

  • Notes

    Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.

    Arf1 (ADP-ribosylation factor 1) is a 21 kDa protein, member of ARF gene family, which constitutes part of the Ras superfamily. The Arf proteis are categorized as class I (Arf1, Arf2, Arf3), class II (Arf4 and Arf5) and class III (Arf6), and they all share a common gene organization. Arf1 regulates a variety of biological response pathways that include vesicle and protein trafficking, organelle structure, and endocytosis. Like other small GTPases, Arf1 cycles between an inactive GDP-bound form and an active GTP-bound form: in its active (GTP-bound) state, Arf1 binds specifically to the protein-binding domain (PBD) of GGA3 to control downstream signaling cascades.

Properties

Associated products

Images

  • Arf1 activation assay. Lane 1: HeLa cell lysate loaded with GDP and incubated with GGA3 PBD Agarose beads. Lane 2: HeLa cell lysate loaded with GTPγS and incubated with GGA3 PBD Agarose beads.

  • Arf1 positive control (293 cell lysate at 0.5 mg/mL) detected by immunoblot.

Protocols

References

ab211170 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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