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Arf1 Activation Assay Kit (ab211170) uses GGA3 PBD Agarose beads to selectively isolate and pull-down the active form of Arf from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Arf is detected by western blot analysis using an anti-Arf1 antibody, which detects the human, mouse and rat protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Arf1 (ADP-ribosylation factor 1) is a 21 kDa protein, member of ARF gene family, which constitutes part of the Ras superfamily. The Arf proteis are categorized as class I (Arf1, Arf2, Arf3), class II (Arf4 and Arf5) and class III (Arf6), and they all share a common gene organization. Arf1 regulates a variety of biological response pathways that include vesicle and protein trafficking, organelle structure, and endocytosis. Like other small GTPases, Arf1 cycles between an inactive GDP-bound form and an active GTP-bound form: in its active (GTP-bound) state, Arf1 binds specifically to the protein-binding domain (PBD) of GGA3 to control downstream signaling cascades.
|Components||20 tests||5 tests|
|ab211180 - GGA3 PBD Agarose Beads||1 x 800µl||1 x 200µl|
|100X GTPyS||1 x 50µl||1 x 20µl|
|100X GDP||1 x 50µl||1 x 20µl|
|5X Assay/Lysis Buffer||1 x 30ml||4 x 2ml|
|anti-Arfl, Mouse monoclonal antibody||1 x 40µl||1 x 10µl|
|GTPase immunoblot Positive Control||1 x 100µl||1 x 100µl|
ab211170 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"