Recombinant Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10429] to Arp3 - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Arp3 antibody [EPR10429] - C-terminal
See all Arp3 primary antibodies -
Description
Rabbit monoclonal [EPR10429] to Arp3 - C-terminal -
Host species
Rabbit -
Specificity
The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat.
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Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, A549, Mouse spleen, Rat spleen, and Mouse brain lysates. IHC-P: human kidney, and mouse kidney tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10429 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified please use at 1/40 |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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ICC/IF |
1/50 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified please use at 1/40 |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The rat recommendation is based on the WB results. We do not guarantee IHC-P for rat. |
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
ICC/IF
1/50 - 1/100. |
Target
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Relevance
The Arp2/3 complex involved in Golgi polerization in NIH3T3 cells. In a different model it has been shown that the Arp2/3 complex is necessary for neutrophil chemotaxis and phagocytosis. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 10096 Human
- Omim: 604222 Human
- SwissProt: P61158 Human
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Alternative names
- Actin like protein 3 antibody
- Actin related protein 3 antibody
- ACTR3 antibody
- ARP3 actin related protein 3 homolog (yeast) antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Arp3 with purified ab181164 at 1:250 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Arp3 with purified ab181164 at 1:50 dilution (5.06 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Arp3 with purified ab181164 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Arp3 with purified ab181164 at 1:250 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Rat spleen lysates
Lane 4 : Mouse brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa -
All lanes : Anti-Arp3 antibody [EPR10429] - C-terminal (ab181164) at 1/10000 dilution (Unpurified)
Lane 1 : HeLa lysate
Lane 2 : Jurkat lysate
Lane 3 : A549 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDa -
Immunofluorescence analysis of -20°C Acetone fixed HeLa cells labeling Arp3 with unpurified ab181164 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution.
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Immunohistochemical analysis of paraffin embedded human kidney tissue labeling Arp3 with unpurified ab181164 at a 1/250 dilution. Prediluted HRP Rabbit IgG secondary was used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab181164 has been referenced in 3 publications.
- Li Y et al. Dragon's Blood Regulates Rac1-WAVE2-Arp2/3 Signaling Pathway to Protect Rat Intestinal Epithelial Barrier Dysfunction Induced by Simulated Microgravity. Int J Mol Sci 22:N/A (2021). PubMed: 33800361
- Liu Y et al. JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice. FEBS J 287:5478-5497 (2020). PubMed: 32279424
- Wu D et al. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Oncotarget 8:88630-88644 (2017). WB ; Mouse . PubMed: 29179463