Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.2 nmol/well
Product nameAscorbic Acid Assay Kit (Biological Samples)
See all Ascorbic acid kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts, Cell culture media
Sensitivity> 0.2 nmol/well
Range0.2 nmol/well - 20 nmol/well
Assay time0h 30m
Ascorbic Acid Assay Kit (Biological Samples) (ab65656) provides a rapid, simple, and sensitive means of detecting ascorbic acid (vitamin C) in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products.
In the ascorbic acid assay protocol, Fe3+ is reduced to Fe2+ by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between OD=545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content.
The assay can detect 0.2 to 20 nmol of ascorbic acid.
Ascorbic acid assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader in kinetic mode every minute for 2-3 minutes
This product is manufactured by BioVision, an Abcam company and was previously called K671 Ascorbic Acid Colorimetric Assay Kit II (FRASC). K671-100 is the same size as the 100 test size of ab65656.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Ascorbate Oxidase (lyophilized) 1 vial Ascorbic Acid Probe 1 x 1ml Ascorbic Acid Standard (Lyophilised) 1 vial FeCl3 solution 1 x 1ml Ferric Reducing Ascorbate Buffer 1 x 25ml
RelevanceAscorbic Acid (C6H8O6) is an essential nutrient in human diets, and is necessary to maintain connective tissue and bone. The biologically active form of ascorbic acid is vitamic C, a water soluble vitamin which is the L-enantiomer form of ascorbic acid. Humans have lost the ability to synthesize ascorbic acid and must obtain in the diet. Ascorbic acid is an electron donor for enzymes involved in collagen hydroxylation, biosynthesis of carnitine and norepinephrine, tyrosine metabolism and amidatin of peptide hormones. The ability of vitamin C to donate electrons also makes it a potent water-soluble anti-oxidant that readily scavenges free radicals.
- Vitamin C
ab65656 has been referenced in 5 publications.
- Chang YL et al. A screen of Crohn's disease-associated microbial metabolites identifies ascorbate as a novel metabolic inhibitor of activated human T cells. Mucosal Immunol 12:457-467 (2019). PubMed: 29695840
- Lee H et al. Ascorbic acid inhibits visceral obesity and nonalcoholic fatty liver disease by activating peroxisome proliferator-activated receptor a in high-fat-diet-fed C57BL/6J mice. Int J Obes (Lond) 43:1620-1630 (2019). PubMed: 30283077
- Perriotte-Olson C et al. Nanoformulated copper/zinc superoxide dismutase reduces adipose inflammation in obesity. Obesity (Silver Spring) 24:148-56 (2016). Functional Studies . PubMed: 26612356
- Blaschke K et al. Vitamin C induces Tet-dependent DNA demethylation and a blastocyst-like state in ES cells. Nature 500:222-6 (2013). PubMed: 23812591
- Dai D et al. Healing of saccular aneurysms following platinum coil embolization: lack of improved efficacy with vitamin C supplementation. J Neurointerv Surg N/A:N/A (2012). Functional Studies ; Rabbit . PubMed: 22914744