Recombinant Anti-Axl antibody [EPR19880] (ab219651)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19880] to Axl
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Axl antibody [EPR19880]
See all Axl primary antibodies -
Description
Rabbit monoclonal [EPR19880] to Axl -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate; 293T transfected with a His-tagged human Axl construct whole cell lysate. IHC-P: Human breast and breast carcinoma, lung and lung carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19880 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/10000. Predicted molecular weight: 98 kDa.
Detection of endogenous expression of AXL in WB will need optimization. Under our experimental conditions we could not detect endogenous AXL in human cell and tissue lysates. |
Notes |
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IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/10000. Predicted molecular weight: 98 kDa. Detection of endogenous expression of AXL in WB will need optimization. Under our experimental conditions we could not detect endogenous AXL in human cell and tissue lysates. |
Target
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Function
May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor. -
Tissue specificity
Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 558 Human
- Omim: 109135 Human
- SwissProt: P30530 Human
- Unigene: 590970 Human
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Alternative names
- Adhesion related kinase antibody
- AI323647 antibody
- Ark antibody
see all
Images
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All lanes : Anti-Axl antibody [EPR19880] (ab219651) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate at 40 µg
Lanes 2 & 4 : AXL knockout HeLa cell lysate at 20 µg
Lane 3 : Wild-type HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 98 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab219651 observed at 135 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab219651 was shown to react with Axl in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab219651 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded human breast and breast carcinoma tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on cancer cells of the human breast carcinoma, and the pericarcinous tissue was nearly negative (magnification x150) (PMID: 25337673).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on human lung.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on cancer cells of human lung carcinoma (PMID: 25337673).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human adipose tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Negative control: No staining on human adipose tissues (PMID: 26573603).
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Axl antibody [EPR19880] (ab219651) at 1/10000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney) transfected with an empty expression vector, whole cell lysate
Lane 2 : 293T (Human epithelial cell line from embryonic kidney) transfected with a His-tagged human Axl construct whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution
Predicted band size: 98 kDa
Observed band size: 138 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Detection of endogenous expression of Axl in WB will need optimization. Under our experimental conditions we could not detect endogenous Axl in human cell and tissue lysates.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab219651 has been referenced in 4 publications.
- Chen A et al. Comprehensive Oncogenic Features of Coronavirus Receptors in Glioblastoma Multiforme. Front Immunol 13:840785 (2022). PubMed: 35464443
- Wang S et al. AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells. Cell Res 31:126-140 (2021). PubMed: 33420426
- Mahadevan NR et al. Intrinsic Immunogenicity of Small Cell Lung Carcinoma Revealed by Its Cellular Plasticity. Cancer Discov 11:1952-1969 (2021). PubMed: 33707236
- Li XL et al. Rapamycin Alleviates the Symptoms of Multiple Sclerosis in Experimental Autoimmune Encephalomyelitis (EAE) Through Mediating the TAM-TLRs-SOCS Pathway. Front Neurol 11:590884 (2020). PubMed: 33329339