Anti-B19 antibody (ab137492)
Key features and details
- Rabbit polyclonal to B19
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-B19 antibody
See all B19 primary antibodies -
Description
Rabbit polyclonal to B19 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human B19 aa 650-714 (C terminal).
Database link: P57058 -
Positive control
- A431 and HeLa whole cell lysate (ab150035); A549 cells; Human OVCA xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer pH 8
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer pH 8 |
ICC/IF
1/100 - 1/1000. |
Target
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Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 30811 Human
- Omim: 606532 Human
- SwissProt: P57058 Human
- Unigene: 109437 Human
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Alternative names
- B19 antibody
- Bstk1 antibody
- Hormonally up-regulated neu tumor-associated kinase antibody
see all
Images
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Anti-B19 antibody (ab137492) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labelling B19 with ab137492 at 1/200 dilution. The lower image is costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue labelling B19 with ab137492 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137492 has been referenced in 1 publication.
- Acevedo L et al. Comparison of Human Articular Cartilage Tissue and Chondrocytes Isolated from Peripheral versus Central Regions of Traumatic Lesions. Cartilage 13:68S-81S (2021). PubMed: 32959685