Overview

  • Product name
  • Description
    Rabbit polyclonal to BACH2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BACH2 aa 791-841. The exact sequence is proprietary.
    Database link: Q9BYV9

  • Positive control
    • WB: HEK-293T and Jurkat cell lysate. IP: HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK.
  • Tissue specificity
    B-cell specific.
  • Sequence similarities
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 1 bZIP domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bach 2 antibody
    • Bach2 antibody
    • BACH2_HUMAN antibody
    • Basic leucine zipper transcription factor 2 antibody
    • BTB and CNC homolog 2 antibody
    • BTB and CNC homology 1 basic leucine zipper transcription factor 2 antibody
    • BTB domain and CNC homolog 2 antibody
    • BTBD25 antibody
    • OTTHUMP00000016867 antibody
    • OTTHUMP00000016868 antibody
    • OTTHUMP00000040591 antibody
    • Transcription regulator protein BACH2 antibody
    see all

Images

  • All lanes : Anti-BACH2 antibody (ab226394) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 3 minutes
  • BACH2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer; 1.0 mg for IP, 20% of IP loaded) with ab226394 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226394 at 1 µg/ml.

    Lane 1: ab226394 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 minutes.

References

ab226394 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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