Anti-BCAT2 antibody (ab95976)
Key features and details
- Rabbit polyclonal to BCAT2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BCAT2 antibody
See all BCAT2 primary antibodies -
Description
Rabbit polyclonal to BCAT2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 25-302 of Human BCAT2 (NP_001181).
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Positive control
- Raji whole cell lysates; A431 cells; Ca922; H1299, HeLaS3, HepG2, Molt4 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Cytoplasm and Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 587 Human
- Entrez Gene: 12036 Mouse
- Omim: 113530 Human
- SwissProt: O15382 Human
- SwissProt: O35855 Mouse
- Unigene: 512670 Human
- Unigene: 24210 Mouse
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Alternative names
- BCAM antibody
- BCAT 2 antibody
- BCAT(m) antibody
see all
Images
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Anti-BCAT2 antibody (ab95976) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 44 kDa
10% SDS-PAGE -
ab95976, at 1/200 dilution, staining BCAT2 in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.
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Paraffin embedded Ca922 tissue stained for BCAT2 using ab95976 at 1/100 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab95976 has been referenced in 7 publications.
- Zhang Y et al. Identification of 14 Differentially-Expressed Metabolism-Related Genes as Potential Targets of Gastric Cancer by Integrated Proteomics and Transcriptomics. Front Cell Dev Biol 10:816249 (2022). PubMed: 35265615
- Siddik MAB et al. Branched-Chain Amino Acids Are Linked with Alzheimer's Disease-Related Pathology and Cognitive Deficits. Cells 11:N/A (2022). PubMed: 36359919
- Wang K et al. Branched-chain amino acid aminotransferase 2 regulates ferroptotic cell death in cancer cells. Cell Death Differ 28:1222-1236 (2021). PubMed: 33097833
- Singh R et al. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases. Diabetes Metab Syndr Obes 11:65-84 (2018). PubMed: 29618935
- Wang WM et al. MicroRNA-182 Regulates Neurite Outgrowth Involving the PTEN/AKT Pathway. Front Cell Neurosci 11:96 (2017). WB ; Mouse . PubMed: 28442995
- Antanaviciute I et al. Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism. Sci Rep 7:7820 (2017). WB ; Human . PubMed: 28798381
- Kitsy A et al. Effects of leucine supplementation and serum withdrawal on branched-chain amino acid pathway gene and protein expression in mouse adipocytes. PLoS One 9:e102615 (2014). WB ; Mouse . PubMed: 25050624