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Our Abpromise guarantee covers the use of ab27171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC/IF||1/200. Fix cells with paraformaldehyde and block with BSA.|
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ab27171 at 1/200 staining human foetal neural precursors by ICC/IF. The cells were paraformaldehyde fixed, blocked with BSA and incubated with the antibody for 12 hours. An Alexa Fluor ® 594 conjugated goat anti-rabbit IgG was used as the secondary. Human neurons and astrocytes derived from neural precursors were used as the negative control. ab27171 has weak non-specific staining of the nucleolus however staining of the cytoplasm has not been detected in negative controls.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"