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Mouse monoclonal [ANa40] to BNIP3 (HRP)
Predicted to work with:
Recombinant fragment corresponding to Human BNIP3 aa 1 to the C-terminus.
The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.
WB: Human skeletal muscle tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Preservative: 0.1% Proclin Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
Light chain type
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 21.5 kDa).
Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Belongs to the NIP3 family.
Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
Information by UniProt
BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody
Western blot - Anti-BNIP3 antibody [ANa40] (HRP) (ab196707)
Anti-BNIP3 antibody [ANa40] (HRP) (ab196707) at 1/5000 dilution + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
30 kDa (
why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196707 overnight at 4°C. Antibody binding was visualised using ECL development solution
has not yet been referenced specifically in any publications.
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