The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 - 4 µg/ml. Predicted molecular weight: 22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Belongs to the NIP3 family.
Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
Chaanine AH et al. JNK modulates FOXO3a for the expression of the mitochondrial death and mitophagy marker BNIP3 in pathological hypertrophy and in heart failure. Cell Death Dis3:265 (2012).
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