Anti-BOP1 antibody (ab86652)
Key features and details
- Rabbit polyclonal to BOP1
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BOP1 antibody
See all BOP1 primary antibodies -
Description
Rabbit polyclonal to BOP1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Pig, Rhesus monkey, Gorilla, Chinese hamster, Bat -
Immunogen
Synthetic peptide corresponding to Human BOP1 aa 350-450.
Database link: Q14137 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. -
Sequence similarities
Belongs to the WD repeat BOP1/ERB1 family.
Contains 7 WD repeats. -
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23246 Human
- Entrez Gene: 12181 Mouse
- Entrez Gene: 300050 Rat
- Omim: 610596 Human
- SwissProt: Q14137 Human
- SwissProt: P97452 Mouse
- SwissProt: Q562C2 Rat
- Unigene: 645279 Human
see all -
Alternative names
- AU020183 antibody
- AW146150 antibody
- Block of proliferation 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse teratoma tissues labelling BOP1 with ab86652 at 1/200 (1µg/ml). Detection: DAB.
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Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86652 at 3 µg/mg lysate; Lane 2 represents control IgG IP. For the WB, ab86653 was used at 0.4 µg/ml.
Detection: Chemiluminescence with exposure time of 90 seconds.
All lanes : Anti-BOP1 antibody (ab86652) at 0.4 µg/ml
Lane 1 : immunoprecipitated BOP1
Lane 2 : control IgG
Observed band size: 101 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 55 kDa, 60-71 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab86652 has been referenced in 2 publications.
- Chen B et al. The Long Noncoding RNA CCAT2 Induces Chromosomal Instability Through BOP1-AURKB Signaling. Gastroenterology 159:2146-2162.e33 (2020). PubMed: 32805281
- Corsini NS et al. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation. Cell Stem Cell 22:543-558.e12 (2018). PubMed: 29625069