Anti-Bcl-2 antibody (ab194583)
Key features and details
- Rabbit polyclonal to Bcl-2
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Bcl-2 antibody
See all Bcl-2 primary antibodies -
Description
Rabbit polyclonal to Bcl-2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Bcl-2 aa 1-100.
Database link: P10415 -
Positive control
- WB: HL-60 cell lysate, mouse testis, rat lung and rat liver tissue lysates; IHC: Human lung cancer, rat brain and mouse testis; ICC/ IF: C6, NIH-3T3 and U-2 OS cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/500 - 1/2000. Predicted molecular weight: 26 kDa.
|
|
ICC/IF |
1/50 - 1/100.
|
|
IP |
1/1000.
|
Notes |
---|
IHC-P
1/50 - 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/50 - 1/100. |
IP
1/1000. |
Target
-
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). -
Tissue specificity
Expressed in a variety of tissues. -
Involvement in disease
A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. -
Post-translational
modificationsPhosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. -
Cellular localization
Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 596 Human
- Entrez Gene: 12043 Mouse
- Entrez Gene: 24224 Rat
- Omim: 151430 Human
- SwissProt: P10415 Human
- SwissProt: P10417 Mouse
- SwissProt: P49950 Rat
- Unigene: 150749 Human
see all -
Alternative names
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
see all
Images
-
All lanes : Anti-Bcl-2 antibody (ab194583) at 1/500 dilution
Lane 1 : 239T cell lysate
Lane 2 : 239T Bcl-2 knock-out cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 26 kDaBlocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
All lanes : Anti-Bcl-2 antibody (ab194583) at 1/500 dilution
Lane 1 : Mouse lung cell extract
Lane 2 : Mouse spleen cell extract
Lane 3 : Rat spleen cell extract
Lane 4 : Rat ovary cell extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 26 kDaBlocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic KitExposure time: 30 seconds
-
Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug Bcl-2 antibody (ab194583). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (ab194583) at a dilition of 1:1000.
-
Immunofluorescence analysis of U-2 OS cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
-
Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
-
Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (ab194583) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (107)
ab194583 has been referenced in 107 publications.
- Liu L et al. Hypercholesterolemia aggravates sevoflurane-induced cognitive impairment in aged rats by inducing neurological inflammation and apoptosis. J Biochem Mol Toxicol 36:e23009 (2022). PubMed: 35174938
- Zhuang J et al. Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner. BMC Cardiovasc Disord 22:69 (2022). PubMed: 35196979
- Shi X et al. Circ_0037866 Contributes to the Tumorigenesis of Renal Cell Carcinoma by Sequestering miR-384 to Elevate Chromobox 5 Expression. Kidney Blood Press Res 47:329-340 (2022). PubMed: 35249038
- Yang Q & Long F MiRNA-384-5p targets GABRB1 to regulate ketamine-Induced Neurotoxicity in Neurons. Turk Neurosurg N/A:N/A (2022). PubMed: 35416273
- Wang S et al. Pathological Changes and Expression of JAK-STAT Signaling Pathway Hallmark Proteins in Rat Retinas at Different Time Points After Retinal Ischemia Reperfusion Injury. Pathol Oncol Res 28:1610385 (2022). PubMed: 35515015