Anti-Bcl3 antibody (ab27780)
Key features and details
- Rabbit polyclonal to Bcl3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl3 antibody
See all Bcl3 primary antibodies -
Description
Rabbit polyclonal to Bcl3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Bcl3. Synthetic peptide surrounding amino acid 424 of human Bcl-3
Database link: P20749 -
Positive control
- WB: Jurkat
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3407 Bcl-3 Antibody. 3407-100 is the same size as the 100 µg size of ab27780.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol (glycerin, glycerine), 0.5% BSA, 69% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
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Notes |
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. -
Involvement in disease
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions. -
Sequence similarities
Contains 7 ANK repeats. -
Post-translational
modificationsPolyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. - Information by UniProt
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Database links
- Entrez Gene: 602 Human
- Omim: 109560 Human
- SwissProt: P20749 Human
- Unigene: 31210 Human
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Alternative names
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab27780 has been referenced in 2 publications.
- Shao M et al. MYBL2 protects against H9c2 injury induced by hypoxia via AKT and NF-?B pathways. Mol Med Rep 17:4832-4838 (2018). PubMed: 29328450
- Long TY et al. CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-?B pathway. Braz J Med Biol Res 50:e5861 (2017). PubMed: 28355355