Recombinant Anti-Beclin 1 antibody [EPR19662] (ab207612)
![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261167-anti-beclin-1-antibody-epr19662-western-blot.jpg "Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19662] to Beclin 1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Beclin 1 antibody [EPR19662]
See all Beclin 1 primary antibodies -
Description
Rabbit monoclonal [EPR19662] to Beclin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, HEK-293, MCF7, Jurkat, Raji, C6, RAW264.7, PC-12, NIH/3T3, A20 and C2C12 whole cell lysates; rat brain, kidney and spleen lysates; mouse brain, heart and spleen lysates. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19662 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (6) |
1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| IP | (1) |
1/30.
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| Notes |
|---|
|
WB
1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
|
IP
1/30. |
Target
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Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the beclin family. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: O88597 Mouse
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
see all -
Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Images
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Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261165-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 30 seconds; Lane 5: 15 seconds; Lane 6: 15 seconds.
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261164-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)Lanes 1-4 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lanes 5-6 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate
Lane 6 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 5 seconds; Lane 5: 10 seconds; Lane 6: 30 seconds.
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261163-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat kidney lysate
Lane 3 : Rat spleen lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/6: 5 seconds; Lane 2/3: 15 seconds; Lane 4: 3 seconds; Lane 5: 10 seconds.
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![Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261162-anti-beclin-1-antibody-epr19662-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)Beclin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab207612 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207612 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10µg (Input).
Lane 2: ab207612 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207612 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-9-benefits-of-recombinant-antibodies.png)
Anti-Beclin 1 antibody [EPR19662] (ab207612)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (153)
ab207612 has been referenced in 153 publications.
- Mirra S et al. CERKL, a retinal dystrophy gene, regulates mitochondrial function and dynamics in the mammalian retina. Neurobiol Dis 156:105405 (2021). WB . PubMed: 34048907
- Diao Y Clematichinenoside AR Alleviates Foam Cell Formation and the Inflammatory Response in Ox-LDL-Induced RAW264.7 Cells by Activating Autophagy. Inflammation 44:758-768 (2021). PubMed: 33151398
- Long C et al. FOXO3 is targeted by miR-223-3p and promotes osteogenic differentiation of bone marrow mesenchymal stem cells by enhancing autophagy. Hum Cell 34:14-27 (2021). PubMed: 32920731
- Wang L et al. LncRNA BCYRN1-induced autophagy enhances asparaginase resistance in extranodal NK/T-cell lymphoma. Theranostics 11:925-940 (2021). PubMed: 33391513
- Su Q et al. Trichostatin A ameliorates Alzheimer's disease-related pathology and cognitive deficits by increasing albumin expression and Aß clearance in APP/PS1 mice. Alzheimers Res Ther 13:7 (2021). PubMed: 33397436