Name: Anti-Beclin 1 antibody [EPR19662]
Brand: RabMAb
SKU: ab207612
Rating: 5 (5 reviews)

Product name

Anti-Beclin 1 antibody [EPR19662]
See all Beclin 1 primary antibodies
Description

Rabbit monoclonal [EPR19662] to Beclin 1
Host species

Rabbit
Tested applications

Suitable for: WB, IP, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human Beclin 1 aa 1-200. The exact sequence is proprietary.
Database link: Q14457

Run BLAST with Run BLAST with
Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, 293, MCF7, Jurkat, Raji, C6, RAW264.7, PC-12, NIH/3T3, A20 and C2C12 whole cell lysates; rat brain, kidney and spleen lysates; mouse brain, heart and spleen lysates. IP: HeLa whole cell lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19662
Isotype

IgG
Research areas

Alternative Versions
- Anti-Beclin 1 antibody [EPR19662] (Alexa Fluor® 488) (ab225466)
- Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free (ab227107)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

Our Abpromise guarantee covers the use of ab207612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP		1/30.
ICC/IF		Use at an assay dependent concentration.

Function

Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Tissue specificity

Ubiquitous.
Sequence similarities

Belongs to the beclin family.
Cellular localization

Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
Target information above from: UniProt accession Q14457 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: O88597 Mouse
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
- Unigene: 178947 Mouse
- Unigene: 2776 Rat
see all
Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
- ATG6 autophagy related 6 homolog antibody
- Bcl-2-interacting protein beclin antibody
- Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
- Beclin 1 autophagy related antibody
- Beclin-1 antibody
- Beclin1 antibody
- BECN 1 antibody
- Becn1 antibody
- BECN1_HUMAN antibody
- Coiled coil myosin like BCL2 interacting protein antibody
- Coiled-coil myosin-like BCL2-interacting protein antibody
- GT197 antibody
- Protein GT197 antibody
- VPS 30 antibody
- VPS30 antibody
see all

Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody [EPR19662] (ab207612)This image is courtesy of an Abreview submitted by Dr Kirk McManus, Univ. of Manitoba/Cancer Care MICB.

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Beclin 1 with ab207612 at 1/200 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton-X100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 22°C. ab150081, a goat anti-rabbit IgG H + L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution.
See Abreview
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-4: 30 seconds; Lane 5: 15 seconds; Lane 6: 15 seconds.
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

Lanes 1-4 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lanes 5-6 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate
Lane 6 : C2C12 (Mouse myoblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-4: 5 seconds; Lane 5: 10 seconds; Lane 6: 30 seconds.
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat kidney lysate
Lane 3 : Rat spleen lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/6: 5 seconds; Lane 2/3: 15 seconds; Lane 4: 3 seconds; Lane 5: 10 seconds.
Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)

Beclin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab207612 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab207612 at 1/1000 dilution.

VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

Lane 1: HeLa whole cell lysate 10µg (Input).

Lane 2: ab207612 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207612 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.

Western blot protocols
Immunoprecipitation protocols
Immunocytochemistry & immunofluorescence protocols

This product has been referenced in:

Svarcbahs R et al. Removal of prolyl oligopeptidase reduces alpha-synuclein toxicity in cells and in vivo. Sci Rep 8:1552 (2018). Read more (PubMed: 29367610) »
Fan T et al. Hypoxia-induced autophagy is inhibited by PADI4 knockdown, which promotes apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis. Mol Med Rep 17:5116-5124 (2018). Read more (PubMed: 29393388) »

See all 18 Publications for this product

Publishing research using ab207612? Please let us know so that we can cite the reference in this datasheet.

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1-5 of 5 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Mouse Cell lysate - whole cell (NIH 3T3 cells)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

10 µg

Specification

NIH 3T3 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 08 2017

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (HeLa cells)

Total protein in input

400 µg

Immuno-precipitation step

Protein A

Specification

HeLa cells

Abcam user community

Verified customer

Submitted Sep 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (LS174t cells)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

20 µg

Specification

LS174t cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 28 2017

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (HepG2 cells)

Gel Running Conditions

Reduced Denaturing (7.5%)

Loading amount

15 µg

Treatment

Different Concentrations of CpdX

Specification

HepG2 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 16 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Yes - 0.5% Triton-X100 in PBS

Specification

HeLa

Fixative

Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Sep 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-Beclin 1 antibody [EPR19662] (ab207612)

Overview

Properties

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Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

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