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    beclin-1-antibody-epr19662-ab207612.pdf

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Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Tumor Suppressors
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RecombinantRabMAb

Recombinant Anti-Beclin 1 antibody [EPR19662] (ab207612)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (10) Submit a question References (153)

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Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)
  • Anti-Beclin 1 antibody [EPR19662] (ab207612)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19662] to Beclin 1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Beclin 1 antibody [EPR19662]
    See all Beclin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19662] to Beclin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, HEK-293, MCF7, Jurkat, Raji, C6, RAW264.7, PC-12, NIH/3T3, A20 and C2C12 whole cell lysates; rat brain, kidney and spleen lysates; mouse brain, heart and spleen lysates. IP: HeLa whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19662
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Immune Response
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free (ab227107)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Beclin 1 protein (ab137161)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (6)
1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP (1)
1/30.
Notes
WB
1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP
1/30.

Target

  • Function

    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the beclin family.
  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Target information above from: UniProt accession Q14457 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8678 Human
    • Entrez Gene: 56208 Mouse
    • Entrez Gene: 114558 Rat
    • Omim: 604378 Human
    • SwissProt: Q14457 Human
    • SwissProt: O88597 Mouse
    • SwissProt: Q91XJ1 Rat
    • Unigene: 716464 Human
    • Unigene: 178947 Mouse
    • Unigene: 2776 Rat
    see all
  • Alternative names

    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Images

  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
    Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
    All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
    Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
    All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 6 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 30 seconds; Lane 5: 15 seconds; Lane 6: 15 seconds.

  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
    Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
    Lanes 1-4 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
    Lanes 5-6 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 5 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate
    Lane 6 : C2C12 (Mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 5 seconds; Lane 5: 10 seconds; Lane 6: 30 seconds.

  • Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
    Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)
    All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Mouse heart lysate
    Lane 6 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/6: 5 seconds; Lane 2/3: 15 seconds; Lane 4: 3 seconds; Lane 5: 10 seconds.

  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)
    Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)

    Beclin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab207612 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab207612 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10µg (Input).

    Lane 2: ab207612 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207612 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Beclin 1 antibody [EPR19662] (ab207612)
    Anti-Beclin 1 antibody [EPR19662] (ab207612)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (153)

Publishing research using ab207612? Please let us know so that we can cite the reference in this datasheet.

ab207612 has been referenced in 153 publications.

  • Mirra S  et al. CERKL, a retinal dystrophy gene, regulates mitochondrial function and dynamics in the mammalian retina. Neurobiol Dis 156:105405 (2021). WB . PubMed: 34048907
  • Diao Y Clematichinenoside AR Alleviates Foam Cell Formation and the Inflammatory Response in Ox-LDL-Induced RAW264.7 Cells by Activating Autophagy. Inflammation 44:758-768 (2021). PubMed: 33151398
  • Long C  et al. FOXO3 is targeted by miR-223-3p and promotes osteogenic differentiation of bone marrow mesenchymal stem cells by enhancing autophagy. Hum Cell 34:14-27 (2021). PubMed: 32920731
  • Wang L  et al. LncRNA BCYRN1-induced autophagy enhances asparaginase resistance in extranodal NK/T-cell lymphoma. Theranostics 11:925-940 (2021). PubMed: 33391513
  • Su Q  et al. Trichostatin A ameliorates Alzheimer's disease-related pathology and cognitive deficits by increasing albumin expression and Aß clearance in APP/PS1 mice. Alzheimers Res Ther 13:7 (2021). PubMed: 33397436
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 10 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Beclin 1 antibody [EPR19662]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (testis cell)
Permeabilization
No
Specification
testis cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Oct 15 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Beclin 1 antibody [EPR19662]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Murine Immune Cell)
Permeabilization
Yes - 0.1% Triton
Specification
Murine Immune Cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 13 2021

Western blot abreview for Anti-Beclin 1 antibody [EPR19662]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Gastric Cancer Cell Line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
5 µg
Specification
Gastric Cancer Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 01 2020

Western blot abreview for Anti-Beclin 1 antibody [EPR19662]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse macrophage cell line)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
5 µg
Specification
Mouse macrophage cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 26 2020

Western blot abreview for Anti-Beclin 1 antibody [EPR19662]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (C42B Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
C42B Prostate cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

DR. Dimitra Kalamida

Verified customer

Submitted Jun 26 2019

Western blot abreview for Anti-Beclin 1 antibody [EPR19662]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
NIH 3T3 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Sep 08 2017

Immunoprecipitation abreview for Anti-Beclin 1 antibody [EPR19662]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
400 µg
Immuno-precipitation step
Protein A
Specification
HeLa cells
Read More

Abcam user community

Verified customer

Submitted Sep 06 2017

Western blot abreview for Anti-Beclin 1 antibody [EPR19662]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (LS174t cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
LS174t cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Aug 28 2017

Western blot abreview for Anti-Beclin 1 antibody [EPR19662]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
15 µg
Treatment
Different Concentrations of CpdX
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Feb 16 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Beclin 1 antibody [EPR19662]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Read More

DR. Kirk Mcmanus

Verified customer

Submitted Sep 30 2016

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