Recombinant Anti-Beclin 1 antibody [EPR19662] (ab207612)
![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261167-anti-beclin-1-antibody-epr19662-western-blot.jpg "Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19662] to Beclin 1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Beclin 1 antibody [EPR19662]
See all Beclin 1 primary antibodies -
Description
Rabbit monoclonal [EPR19662] to Beclin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, HEK-293, MCF7, Jurkat, Raji, C6, RAW264.7, PC-12, NIH/3T3, A20 and C2C12 whole cell lysates; rat brain, kidney and spleen lysates; mouse brain, heart and spleen lysates. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19662 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Mouse
Rat
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (6) |
1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
|
| IP | (1) |
1/30.
|
| Notes |
|---|
|
WB
1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
|
IP
1/30. |
Target
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Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the beclin family. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: O88597 Mouse
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
see all -
Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Images
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Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261165-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 30 seconds; Lane 5: 15 seconds; Lane 6: 15 seconds.
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261164-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)Lanes 1-4 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lanes 5-6 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate
Lane 6 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 5 seconds; Lane 5: 10 seconds; Lane 6: 30 seconds.
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261163-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat kidney lysate
Lane 3 : Rat spleen lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/6: 5 seconds; Lane 2/3: 15 seconds; Lane 4: 3 seconds; Lane 5: 10 seconds.
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![Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-261162-anti-beclin-1-antibody-epr19662-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)Beclin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab207612 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207612 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10µg (Input).
Lane 2: ab207612 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207612 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-Beclin 1 antibody [EPR19662] (ab207612)](https://www.abcam.com/ps/products/207/ab207612/Images/ab207612-9-benefits-of-recombinant-antibodies.png)
Anti-Beclin 1 antibody [EPR19662] (ab207612)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (81)
ab207612 has been referenced in 81 publications.
- Qi H et al. MSTN Attenuates Cardiac Hypertrophy through Inhibition of Excessive Cardiac Autophagy by Blocking AMPK /mTOR and miR-128/PPAR?/NF-?B. Mol Ther Nucleic Acids 19:507-522 (2020). PubMed: 31923740
- Xia H et al. ube3d, a New Gene Associated with Age-Related Macular Degeneration, Induces Functional Changes in Both In Vivo and In Vitro Studies. Mol Ther Nucleic Acids 20:217-230 (2020). PubMed: 32200270
- Wei M et al. Genipin Induces Autophagy and Suppresses Cell Growth of Oral Squamous Cell Carcinoma via PI3K/AKT/MTOR Pathway. Drug Des Devel Ther 14:395-405 (2020). PubMed: 32099325
- Zhu K et al. LncRNA Sox2OT-V7 promotes doxorubicin-induced autophagy and chemoresistance in osteosarcoma via tumor-suppressive miR-142/miR-22. Aging (Albany NY) 12:6644-6666 (2020). PubMed: 32302291
- Vu M et al. Doxorubicin selectively induces apoptosis through the inhibition of a novel isoform of Bcl-2 in acute myeloid leukaemia MOLM-13 cells with reduced Beclin 1 expression. Int J Oncol 57:113-121 (2020). PubMed: 32377726