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17 amino acid synthetic peptide from near the amino terminus of human Beclin 1
Our Abpromise guarantee covers the use of ab62557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
Paraffin-embedded human brain tissue stained for Beclin 1 using ab62557 at 2.5 µg/ml in immunohistochemical analysis.
ab62557 staining Beclin 1 in Human MCR5 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 21°C. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
ab62557 staining Beclin 1 in Human umbilical vascular endothelial cells after 2 Gy of ionizing radiationby at 4 and 8 days post-irradiation by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3.7% formaldehyde/PBS pH 7.4 for 20 minutes at 37°C, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% PBS with BSA pH 7.4. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. An undiluted Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
Paraffin-embedded rat brain tissue stained for Beclin 1 using ab62557 at 2 µg/ml in immunohistochemical analysis.
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