Anti-Beta Lactamase antibody [8A5.A10] (ab12251)
Key features and details
- Mouse monoclonal [8A5.A10] to Beta Lactamase
- Suitable for: ELISA, WB
- Reacts with: Escherichia coli
- Isotype: IgG1
Overview
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Product name
Anti-Beta Lactamase antibody [8A5.A10] -
Description
Mouse monoclonal [8A5.A10] to Beta Lactamase -
Host species
Mouse -
Specificity
This antibody specifically recognizes TEM type beta lactamases. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant full length protein corresponding to Mouse Beta Lactamase.
Database link: P62593 -
General notes
Dilute in PBS or medium which is identical to that used in the assay system.
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
8A5.A10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use a concentration of 10 - 20 µg/ml.
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WB |
Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa.
(31.5kDa is the molecular weight of the unprocessed precursor.) |
Notes |
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ELISA
Use a concentration of 10 - 20 µg/ml. |
WB
Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa. (31.5kDa is the molecular weight of the unprocessed precursor.) |
Target
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Relevance
The beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant. -
Alternative names
- AmpA antibody
- AmpC antibody
- Cephalosporinase antibody
Datasheets and documents
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Datasheet download
References (27)
ab12251 has been referenced in 27 publications.
- Chaoprasid P et al. Crystal structure of bacterial cytotoxic necrotizing factor CNFY reveals molecular building blocks for intoxication. EMBO J 40:e105202 (2021). PubMed: 33410511
- Naha A et al. Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. PLoS Negl Trop Dis 14:e0008128 (2020). PubMed: 32236098
- Rivas ZP et al. CexE Is a Coat Protein and Virulence Factor of Diarrheagenic Pathogens. Front Microbiol 11:1374 (2020). PubMed: 32714302
- Xiong D et al. Salmonella Coiled-Coil- and TIR-Containing TcpS Evades the Innate Immune System and Subdues Inflammation. Cell Rep 28:804-818.e7 (2019). PubMed: 31315056
- Singh R et al. A ß-lactamase-producing plasmid from Neisseria gonorrhoeae carrying a unique 6 bp deletion in blaTEM-1 encoding a truncated 24 kDa TEM-1 penicillinase that hydrolyses ampicillin slowly. J Antimicrob Chemother 74:2904-2912 (2019). PubMed: 31335939