Beta-Secretase (BACE1) Activity Assay Kit (Fluorometric) (ab282921)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.2 µU
Overview
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Product name
Beta-Secretase (BACE1) Activity Assay Kit (Fluorometric)
See all BACE1 kits -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.2 µU -
Product overview
This Beta-Secretase (BACE1) Activity Assay Kit (Fluorometric), (ab282921) provides a fast and easy method for measuring BACE1 activity in a variety of samples. In this kit, a secretase-specific peptide is conjugated with two reporter molecules, EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by BACE1 separates EDANS and DABCYL groups allowing for the release of a strong fluorescent signal (Ex/Em= 345/500 nm). The level of secretase activity in samples is proportional to the level of fluorescence intensity. The assay is simple, sensitive, high–throughput adaptable and can detect BACE1 activity as low as 0.2 µU per sample.
Species activity: Eukaryotes
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K388 β-Secretase (BACE1) Activity Assay Kit (Fluorometric). K388-100 is the same size as the 100 test size of ab282921.
β-Secretase (EC 3.4.23.46), also called BACE1 (β-site of APP cleaving enzyme) or memapsin-2 (membrane-associated aspartic protease), is an aspartic-acid protease. Along with γ-secretase, BACE1 cleaves the APP (Membrane-anchored amyloid precursor protein) and generates soluble amyloid-β (Aβ). Accumulation of Aβ is the key event for pathogenesis of Alzheimer's disease, thus monitoring and targeting BACE1 activity are important for the study and development of therapeutic strategies that could alleviate/cure Alzheimer’s disease. Recent studies have shown that inhibiting BACE1 activity with synthetic compounds helped to slow or arrest Alzheimer’s disease symptoms.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests BACE1 Assay Buffer WM 1 x 10ml BACE1 Extraction Buffer NM 1 x 25ml BACE1 Inhibitor Control (in DMSO) Blue 1 x 100µl BACE1 Positive Control Red 1 x 20µl BACE1 Substrate (in DMSO) Amber 1 x 200µl EDANS Standard (100 µM) Yellow 1 x 100µl -
Research areas
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Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
Sequence similarities
Belongs to the peptidase A1 family. -
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
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Alternative names
- APP beta secretase
- Asp 2
- ASP2
see all
Images
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BACE1 Activity
Relative BACE1 Activity was calculated in lysates (4 µg) prepared from mouse whole brain (Sample 1) and mouse whole brain (Sample 2). Assays were performed following the kit protocol.
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Kinetic Measurement of BACE1 ActivityKinetic measurement of BACE1 activity in mouse whole brain lysate (4 µg).
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Standard CurveEDANS Standard Curve.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab282921 has not yet been referenced specifically in any publications.