Alkaline Phosphatase Anti-Biotin antibody (ab6652)
Key features and details
- Alkaline Phosphatase Goat polyclonal to Biotin
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Overview
-
Product name
Alkaline Phosphatase Anti-Biotin antibody
See all Biotin primary antibodies -
Description
Alkaline Phosphatase Goat polyclonal to Biotin -
Host species
Goat -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule conjugated to KLH
-
General notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot |
Use at an assay dependent dilution.
|
|
ELISA |
Use at an assay dependent dilution.
|
|
IHC-P |
Use at an assay dependent dilution.
|
|
IHC-Fr |
Use at an assay dependent dilution.
|
|
WB |
1/1000 - 1/3000.
|
Notes |
---|
Dot blot
Use at an assay dependent dilution. |
ELISA
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
WB
1/1000 - 1/3000. |
Target
-
Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab6652 has been referenced in 1 publication.
- Paul A et al. Streptavidin-coated magnetic beads for DNA strand separation implicate a multitude of problems during cell-SELEX. Oligonucleotides 19:243-54 (2009). WB . PubMed: 19732022