BrdU Cell Proliferation Assay Kit (ab287841)
Overview
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Product name
BrdU Cell Proliferation Assay Kit
See all BrdU kits -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Assay duration
Multiple steps standard assay -
Product overview
BrdU Cell Proliferation Assay Kit (ab287841) detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells.
Applications:
- Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients.
- Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.
- Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 200 tests 1000 tests 10X Wash buffer NM/blue cap 1 x 40ml 1 x 200ml 2000X Anti-Mouse HRP-linked Antibody Orange cap 1 x 10µl 1 x 50µl Antibody Diluent NM cap 1 x 50ml 1 x 250ml 1000X BrdU Amber cap 1 x 20µl 1 x 100µl 300X BrdU Detection Antibody Green cap 1 x 70µl 1 x 350µl Fixing/Denaturing Solution WM cap 1 x 20ml 1 x 100ml Stop Solution NM cap 1 x 20ml 1 x 100ml TMB Substrate Amber/NM cap 1 x 20ml 1 x 100ml -
Research areas
Images
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BrdU Cell Proliferation Assay Kit
Example data showing the treatment of Balb/3T3cells with h-FGF2 increases cell proliferation as detected by BrdU Cell Proliferation Assay (ab287841). The cells were seeded at 5000 cells/well in a 96-well plate and incubated overnight at 37°C followed by an overnight incubation in serum-free media at 37°C. h-FGF2 was then added into the plate at different concentrations and cells were incubated for 24 hrs at 37°C. 1X BrdU solution was added into each well and the cells were further incubated for 4 hrs
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab287841 has not yet been referenced specifically in any publications.