Anti-Brk/PTK6 (phospho Y447) antibody (ab138368)
Key features and details
- Rabbit polyclonal to Brk/PTK6 (phospho Y447)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Brk/PTK6 (phospho Y447) antibody
See all Brk/PTK6 primary antibodies -
Description
Rabbit polyclonal to Brk/PTK6 (phospho Y447) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Brk/PTK6 (C terminal) (phospho Y447).
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Positive control
- Extract of 293 cells treated with EGF
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab138368 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 52 kDa. |
Target
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Function
Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression. -
Tissue specificity
Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme. -
Cellular localization
Cytoplasm. Nucleus. Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors. - Information by UniProt
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Database links
- Entrez Gene: 5753 Human
- Omim: 602004 Human
- SwissProt: Q13882 Human
- Unigene: 51133 Human
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Alternative names
- Breast tumor kinase antibody
- Breast tumour kinase antibody
- Protein tyrosine kinase 6 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab138368 has been referenced in 3 publications.
- Gilic MB & Tyner AL Targeting protein tyrosine kinase 6 in cancer. Biochim Biophys Acta Rev Cancer 1874:188432 (2020). PubMed: 32956764
- Wozniak DJ et al. PTEN is a protein phosphatase that targets active PTK6 and inhibits PTK6 oncogenic signaling in prostate cancer. Nat Commun 8:1508 (2017). PubMed: 29142193
- Mathur PS et al. Kinase-Dependent and -Independent Roles for PTK6 in Colon Cancer. Mol Cancer Res 14:563-73 (2016). PubMed: 26983689