Anti-C4a antibody (ab66790)
Key features and details
- Rabbit polyclonal to C4a
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-C4a antibody
See all C4a primary antibodies -
Description
Rabbit polyclonal to C4a -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human C4a aa 700-800 conjugated to keyhole limpet haemocyanin.
(Peptide available asab86260) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa). |
Target
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Function
C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
Involvement in disease
Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis. -
Sequence similarities
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
Post-translational
modificationsPrior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 100293534 Human
- Entrez Gene: 100507685 Human
- Entrez Gene: 720 Human
- Entrez Gene: 721 Human
- Omim: 120810 Human
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
- Unigene: 534847 Human
see all -
Alternative names
- Acidic complement C4 antibody
- C3 and PZP like alpha 2 macroglobulin domain containing protein 2 antibody
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
see all
Images
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Anti-C4a antibody (ab66790) at 1 µg/ml + Hep G2 (Human) Whole Cell Lysate (ab52257) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 193 kDa
Observed band size: 193 kDa -
Anti-C4a antibody (ab66790) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 193 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 41 kDa. We are unsure as to the identity of these extra bands.
Complement C4-A is proteolytically cleaved to yield three non-identical chains and circulates in blood as a disulphide-linked trimer of an alpha, beta and gamma chain (95, 75 and 33 kDa respectively). We propose that the 95 kDa band corresponds to the alpha chain of Complement C4-A.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab66790 has been referenced in 4 publications.
- Lee JH et al. Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis. Clin Proteomics 17:20 (2020). PubMed: 32518534
- Cheng CW et al. Sex hormone-binding globulin (SHBG) is a potential early diagnostic biomarker for gastric cancer. Cancer Med 7:64-74 (2018). WB . PubMed: 29148252
- Song YN et al. Complement C4a inhibits the secretion of hepatitis B virus screened by surface-enhanced laser desorption ionization time-flight mass spectrometry-based ProteinChip analysis. Proteomics Clin Appl 9:1097-104 (2015). PubMed: 26119402
- Treutlein G et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . PubMed: 23236492