• Product name

  • Description

    Rabbit polyclonal to C5a-R
  • Host species

  • Specificity

    This antibody detects endogenous levels of total C5a-R protein.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human C5a-R. non-phosphopeptide derived from human C5a-R around the phosphorylation site of serine 338.

  • Positive control

    • WB: Extracts from HeLa cells treated with PMA (125ng/ml, 30mins).



Our Abpromise guarantee covers the use of ab59390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: 1/5000.
    IHC-P: Use at a concentration of 1 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 39 kDa).
    Lysis buffer: 50mM Tris(pH7.4),150mM NaCl,1% Triton X-100,1% sodium deoxycholate,0.1% SDS and sodium orthovanadate,sodium fluoride,EDTA ,leupeptin.
    Blocking buffer: 3%BSA, 2 hours. Dilute the primary antibody in blocking buffer.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Post-translational

      Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C5a anaphylatoxin chemotactic receptor antibody
      • C5a anaphylatoxin receptor antibody
      • C5A antibody
      • C5a ligand antibody
      • C5a-R antibody
      • C5aR antibody
      • C5AR_HUMAN antibody
      • c5ar1 antibody
      • C5R1 antibody
      • CD88 antibody
      • CD88 antigen antibody
      • Complement component 5 receptor 1 antibody
      • Complement component 5 receptor 2 antibody
      • Complement component 5a receptor 1 antibody
      see all


    • All lanes : Anti-C5a-R antibody (ab59390) at 1/500 dilution

      Lane 1 : Extracts from HeLa cells, treated
      with PMA (125ng/ml, 30mins).
      Lane 2 : Extracts from HeLa cells, treated
      with PMA (125ng/ml, 30mins) plus peptide.

      Predicted band size: 39 kDa
      Observed band size: 46 kDa
      why is the actual band size different from the predicted?

    • ab59390 staining C5a-R in human lung.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.


    This product has been referenced in:

    • Liu X  et al. Panton-Valentine Leukocidin Colocalizes with Retinal Ganglion and Amacrine Cells and Activates Glial Reactions and Microglial Apoptosis. Sci Rep 8:2953 (2018). Read more (PubMed: 29440661) »
    • Zhang XY  et al. Anaphylatoxin C5a induces inflammation and reduces insulin sensitivity by activating TLR4/NF-kB/PI3K signaling pathway in 3T3-L1 adipocytes. Biomed Pharmacother 103:955-964 (2018). WB . Read more (PubMed: 29710512) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Western blot
    Human Cell lysate - whole cell (APRE19 cell line)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    20 µg
    APRE19 cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Lei Wang

    Verified customer

    Submitted Aug 09 2011

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