Recombinant Anti-CAND1 antibody [EPR14241] - N-terminal (ab183748)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14241] to CAND1 - N-terminal
- Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CAND1 antibody [EPR14241] - N-terminal
See all CAND1 primary antibodies -
Description
Rabbit monoclonal [EPR14241] to CAND1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, 293T, Saos-2 whole cell lysates, paraffin-embedded Human pancreas tissue. 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14241 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/50 - 1/70.
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Flow Cyt (Intra) |
1/220.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 136 kDa (predicted molecular weight: 136 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/50 - 1/70. |
Flow Cyt (Intra)
1/220. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Enhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins. -
Sequence similarities
Belongs to the CAND family.
Contains 27 HEAT repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55832 Human
- Entrez Gene: 71902 Mouse
- Entrez Gene: 117152 Rat
- Omim: 607727 Human
- SwissProt: Q86VP6 Human
- SwissProt: Q6ZQ38 Mouse
- SwissProt: P97536 Rat
- Unigene: 546407 Human
see all -
Alternative names
- CAND 1 antibody
- Cand1 antibody
- CAND1_HUMAN antibody
see all
Images
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All lanes : Anti-CAND1 antibody [EPR14241] - N-terminal (ab183748) at 1/20000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : Saos-2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 136 kDa -
Immunohistochemical analysis of Paraffin-embedded Human pancreas tissue labeling CAND1 with ab183748 at 1/100 dilution. HRP conjugated Rabbit IgG was used as a secondary antibody. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 293T cells (fixed in 2% paraformaldehyde) labeling CAND1 with ab183748at 1/220 dilution (red).
Isotype control Rabbit monoclonal IgG (green). Secondary antibody Goat anti rabbit IgG (FITC) at a 1/150 dilution.
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Western blot analysis of CAND1 in immunoprecipitation pellets from Jurkat lysate. ab183748 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab183748 has not yet been referenced specifically in any publications.