Anti-CBF antibody (ab89034)
Key features and details
- Mouse polyclonal to CBF
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CBF antibody
See all CBF primary antibodies -
Description
Mouse polyclonal to CBF -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human CBF. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: NIH/3T3 cell lysate; K-562 cell lysate; CBF transfected 293T cell lysate ICC/IF: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Stimulates transcription from the HSP70 promoter. -
Sequence similarities
Belongs to the CBF/MAK21 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10153 Human
- Entrez Gene: 12607 Mouse
- Omim: 612828 Human
- SwissProt: Q03701 Human
- SwissProt: P53569 Mouse
- Unigene: 135406 Human
- Unigene: 24169 Mouse
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Alternative names
- CBF antibody
- CBF2 antibody
- CCAAT binding factor antibody
see all
Images
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Anti-CBF antibody (ab89034) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg
Predicted band size: 121 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Anti-CBF antibody (ab89034) at 1 µg/ml + K-562 cell lysate at 50 µg
Predicted band size: 121 kDa
Observed band size: 131 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CBF antibody (ab89034) at 1 µg/ml
Lane 1 : CEBPZ transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 121 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted? -
ab89034, at 10 µg/ml, staining CBF in Hela cell by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab89034 has not yet been referenced specifically in any publications.