Product nameAnti-CBFb antibody [EPR6321]
See all CBFb primary antibodies
DescriptionRabbit monoclonal [EPR6321] to CBFb
Tested applicationsSuitable for: WB, ICCmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human CBFb aa 1-100. The exact sequence is proprietary.
- K562, Jurkat and HUVEC cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.97 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
|ICC||1/100 - 1/250.|
FunctionCBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
Involvement in diseaseNote=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
Sequence similaritiesBelongs to the CBF-beta family.
- Information by UniProt
- CBF b antibody
- CBF beta antibody
- CBF-beta antibody
All lanes : Anti-CBFb antibody [EPR6321] (ab124693) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124693 has not yet been referenced specifically in any publications.