Overview

  • Product name
    Anti-CBR1 antibody [EPR9661(B)]
    See all CBR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9661(B)] to CBR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CBR1 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P16152

  • Positive control
    • 293T, HeLa, MCF-7 and HepG2 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
    • Sequence similarities
      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 15 hydroxyprostaglandin dehydrogenase [NADP+] antibody
      • 15-hydroxyprostaglandin dehydrogenase [NADP+] antibody
      • Carbonyl reductase [NADPH] 1 antibody
      • Carbonyl Reductase 1 antibody
      • CBR 1 antibody
      • CBR1 antibody
      • CBR1_HUMAN antibody
      • CRN antibody
      • NADPH dependent carbonyl reductase 1 antibody
      • NADPH-dependent carbonyl reductase 1 antibody
      • Prostaglandin 9 ketoreductase antibody
      • Prostaglandin 9-ketoreductase antibody
      • Prostaglandin E(2) 9 reductase antibody
      • Prostaglandin-E(2) 9-reductase antibody
      • SDR21C1 antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1XPBS (negative control) using ab174852, and HRPconjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • All lanes : Anti-CBR1 antibody [EPR9661(B)] (ab174852) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 30 kDa

    References

    This product has been referenced in:
    • Tak E  et al. Epigallocatechin-3-gallate protects against hepatic ischaemia-reperfusion injury by reducing oxidative stress and apoptotic cell death. J Int Med Res 44:1248-1262 (2016). Read more (PubMed: 27807255) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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