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Our Abpromise guarantee covers the use of ab4148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 30 kDa.Can be blocked with Human CBR1 peptide (ab22991).|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
Detected by chemiluminescence.
Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"