Anti-CCDC65 antibody (ab122482)
Key features and details
- Rabbit polyclonal to CCDC65
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CCDC65 antibody -
Description
Rabbit polyclonal to CCDC65 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: SSVLTPKEQE GIQKNNLEEL TEELTKVMVD YIGMENFWKR YNKVKLEQLS LQHRRAQLLD INGKLREMLK QYLDGISVSD EVLSQ, corresponding to amino acids 368-452 of Human CCDC65 (Q8IXS2).
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Positive control
- Human prostate tissue. Human Plasma lysate. Human tonsil tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/250 - 1/500.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Tissue specificity
Highly expressed in adult testis, in spermatocytes and spermatids. Also observed in spermatogonia. Not detected in Leydig cells, nor in fetal testis (at protein level). - Information by UniProt
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Database links
- Entrez Gene: 85478 Human
- Omim: 611088 Human
- SwissProt: Q8IXS2 Human
- Unigene: 512805 Human
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Alternative names
- CCD65_HUMAN antibody
- CCDC65 antibody
- Coiled coil domain containing 65 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab122482 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122482 at 1/200 dilution staining CCDC65 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
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All lanes : Anti-CCDC65 antibody (ab122482) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122482 has not yet been referenced specifically in any publications.