Anti-CCDC98 antibody (ab204307)
Key features and details
- Rabbit polyclonal to CCDC98
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CCDC98 antibody
See all CCDC98 primary antibodies -
Description
Rabbit polyclonal to CCDC98 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CCDC98 aa 300 to the C-terminus.
Database link: Q6UWZ7 -
Positive control
- IHC-P: Human testis tissue, tonsils tissue. WB: RT4 and U-251 MG cell lysate. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as a central scaffold protein that assembles the various components of the BRCA1-A complex and mediates the recruitment of BRCA1. -
Sequence similarities
Belongs to the FAM175 family. Abraxas subfamily. -
Domain
The MPN-like region is similar to the MPN (JAB/Mov34) domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes. -
Post-translational
modificationsPhosphorylation of Ser-406 of the pSXXF motif by ATM or ATR constitutes a specific recognition motif for the BRCT domain of BRCA1. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Localizes at sites of DNA damage at double-strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 84142 Human
- Omim: 611143 Human
- SwissProt: Q6UWZ7 Human
- Unigene: 334772 Human
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Alternative names
- 3830405G04Rik antibody
- 5630400M01Rik antibody
- ABRA1 antibody
see all
Images
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All lanes : Anti-CCDC98 antibody (ab204307) at 1/100 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Predicted band size: 47 kDa -
Immunofluorescent analysis of U-2 OS cells (PFA-fixed/Triton X-100 permeabilized) labeling CCDC98 with ab204307 at 4 µg/mL.
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Paraffin embedded human testis tissue stained for CCDC98 using ab204307 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human tonsils tissue stained for CCDC98 using ab204307 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human liver tissue stained for CCDC98 using ab204307 at 1/500 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human pancreas tissue stained for CCDC98 using ab204307 at 1/500 dilution in immunohistochemical analysis. Shows no positivity in exocrine glandular cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204307 has not yet been referenced specifically in any publications.