Anti-CCK1-R antibody (ab77269)
Key features and details
- Goat polyclonal to CCK1-R
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CCK1-R antibody
See all CCK1-R primary antibodies -
Description
Goat polyclonal to CCK1-R -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CCK1-R aa 242-257 (internal sequence).
Sequence:C-FEASQKKSAKERKPST
Database link: NP_000721.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab77269 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 886 Human
- Omim: 118444 Human
- SwissProt: P32238 Human
- Unigene: 129 Human
-
Alternative names
- CCK A antibody
- CCK A receptor antibody
- CCK AR antibody
see all
Images
-
ab77269 (4 µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
-
ab77269 at 10 µg/ml staining CCK1-R in human pancreas by Immunohistochemistry, Paraffin-embedded tissue (note cytoplasmic staining of some cells in the islets of Langerhans).
Protocols
Datasheets and documents
-
Datasheet download
References (2)
ab77269 has been referenced in 2 publications.
- Huang L et al. Changes in the interstitial cells of Cajal in the gallbladder of guinea pigs fed a lithogenic diet. Exp Ther Med 22:823 (2021). PubMed: 34131446
- El-Kouhen K & Morisset J Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line. Pancreas 39:127-34 (2010). ICC/IF . PubMed: 19959964