Anti-CD109 antibody [B-E47] - BSA and Azide free (ab47169)
Key features and details
- Mouse monoclonal [B-E47] to CD109 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD109 antibody [B-E47] - BSA and Azide free
See all CD109 primary antibodies -
Description
Mouse monoclonal [B-E47] to CD109 - BSA and Azide free -
Host species
Mouse -
Specificity
Recognises a 170 kDa protein.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD109. Eahy 926 cell line
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Positive control
- Eahy 926 cell line
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General notes
This product was changed from ascites to tissue culture supernatant on 4th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified by affinity chromatography. -
Clonality
Monoclonal -
Clone number
B-E47 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Modulates negatively TGFB1 signaling in keratinocytes. -
Tissue specificity
Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 5 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta. -
Sequence similarities
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
Post-translational
modificationsN-glycosylated.
2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 135228 Human
- Omim: 608859 Human
- SwissProt: Q6YHK3 Human
- Unigene: 399891 Human
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Alternative names
- 150 KDa TGF beta 1 binding protein antibody
- 150 KDa TGF-beta-1-binding protein antibody
- 7D1 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab47169 has not yet been referenced specifically in any publications.