Product nameAnti-CD11b antibody [EPR1344] - Low endotoxin, Azide free
See all CD11b primary antibodies
DescriptionRabbit monoclonal [EPR1344] to CD11b - Low endotoxin, Azide free
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to residues in Human CD11b (UniProt P11215).
- THP1 cell lysate treated with TPA, and TF1 cell lysate; Human tonsil and spleen tissues
ab216445 is a PBS-only buffer formulated version of ab133357, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab133357 for information on protocols, dilutions, and image data.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 127 kDa.|
FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
Tissue specificityPredominantly expressed in monocytes and granulocytes.
Involvement in diseaseGenetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
This IHC data was generated using the same anti-CD11b antibody clone, EPR1344, in a different buffer formulation (cat# ab133357).
IHC image of CD11b staining in a formalin fixed, paraffin embedded human normal spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab133357 at 1/4000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-CD11b antibody [EPR1344] (ab133357) at 1/1000 dilution
Lane 1 : TF-1 cell lysate
Lane 2 : TPA treated THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 127 kDa
This WB data was generated using the same anti-CD11b antibody clone, EPR1344, in a different buffer formulation (cat# ab133357).
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
ab216445 has not yet been referenced specifically in any publications.