Western blot abreview for Anti-CD11b antibody [EPR1344]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Nerve)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
15 µg
Specification
Nerve
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk 5%

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
Nerve before injury
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 160 kDa
Positive control
Nerve after injury

Additional data

Additional Notes
Lane1 and 3: Uninjured Lane 2 and 4: Injured

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Submitted Mar 29 2018

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