Overview

  • Product name
    Anti-CD133 antibody [EPR16508]
    See all CD133 primary antibodies
  • Description
    Rabbit monoclonal [EPR16508] to CD133
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Recombinant fragment within Human CD133 aa 250-400. The exact sequence is proprietary.
    Database link: O43490

  • Positive control
    • WB: Caco-2 (Human colorectal adenocarcinoma epithelial cell), NCCIT (Human pluripotent embryonic carcinoma epithelial cell) and HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate. Human fetal pancreas and fetal kidney lysate. IHC-P: Human pancreas and kidney tissue. Human bladder cancer tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 97 kDa.
IHC-P 1/1000.

Target

  • Function
    May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
  • Tissue specificity
    Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
  • Involvement in disease
    Retinitis pigmentosa 41
    Cone-rod dystrophy 12
    Stargardt disease 4
    Retinal macular dystrophy 2
  • Sequence similarities
    Belongs to the prominin family.
  • Post-translational
    modifications
    Isoform 1 and isoform 2 are glycosylated.
    Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
  • Cellular localization
    Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • AC133 antibody
    • Antigen AC133 antibody
    • CD133 antibody
    • CORD12 antibody
    • Hematopoietic stem cell antigen antibody
    • hProminin antibody
    • MCDR2 antibody
    • MSTP061 antibody
    • OTTHUMP00000217744 antibody
    • OTTHUMP00000217745 antibody
    • OTTHUMP00000217746 antibody
    • PROM1 antibody
    • PROM1_HUMAN antibody
    • Prominin I antibody
    • Prominin like 1 antibody
    • Prominin like protein 1 precursor antibody
    • Prominin mouse like 1 antibody
    • Prominin-1 antibody
    • Prominin-like protein 1 antibody
    • Prominin1 antibody
    • PROML1 antibody
    • RP41 antibody
    • STGD4 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with ab222782 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP)(ready use). Apical/endoluminal staining of human pancreatic ducts, and negative on Langerhans cells of the islets (PMID: 18261235). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)(ready to use).

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD133 with ab222782 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ready to use). Membranous staining on parietal layer of Bowman’s capsule and apical staining on renal tubules in human kidney (PMID: 19092120). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ready to use).

  • All lanes : Anti-CD133 antibody [EPR16508] (ab222782) at 1/10000 dilution

    Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa


    Exposure time: 5 seconds


    Negative control: HeLa (PMID 9389721).

    The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).

    Blocking buffer: 5% NFDM/TBST.

  • Anti-CD133 antibody [EPR16508] (ab222782) at 1/10000 dilution + NCCIT (Human pluripotent embryonic carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa


    Exposure time: 15 seconds


    Blocking buffer: 5% NFDM/TBST.

    The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).

  • All lanes : Anti-CD133 antibody [EPR16508] (ab222782) at 1/2000 dilution

    Lane 1 : Human fetal pancreas lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa


    Exposure time: 3 minutes


    Blocking buffer: 5% NFDM/TBST.

    The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).

  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CD133 with ab222782 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ready to use). Membranous staining on human bladder cancer tumor cells (PMID: 22899341). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ready to use).

References

This product has been referenced in:
  • Wang J  et al. Neural stem cells promote glioblastoma formation in nude mice. Clin Transl Oncol N/A:N/A (2019). ICC/IF . Read more (PubMed: 30945128) »
  • Liu F  et al. Prolonged inhibition of class I PI3K promotes liver cancer stem cell expansion by augmenting SGK3/GSK-3ß/ß-catenin signalling. J Exp Clin Cancer Res 37:122 (2018). Read more (PubMed: 29940988) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Nov 30 2017

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