Overview

  • Product name
    Anti-CD133 antibody [mAbcam27699]
    See all CD133 primary antibodies
  • Description
    Mouse monoclonal [mAbcam27699] to CD133
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse CD133.

    Read Abcam's proprietary immunogen policy (Peptide available as ab31714.)

  • Positive control
    • This antibody gave a positive signal in western blot in the following lysates: Caco-2, HT-29 and mouse kidney normal tissue lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab27699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).

Target

  • Function
    May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
  • Tissue specificity
    Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
  • Involvement in disease
    Retinitis pigmentosa 41
    Cone-rod dystrophy 12
    Stargardt disease 4
    Retinal macular dystrophy 2
  • Sequence similarities
    Belongs to the prominin family.
  • Post-translational
    modifications
    Isoform 1 and isoform 2 are glycosylated.
    Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
  • Cellular localization
    Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • AC133 antibody
    • Antigen AC133 antibody
    • CD133 antibody
    • CORD12 antibody
    • Hematopoietic stem cell antigen antibody
    • hProminin antibody
    • MCDR2 antibody
    • MSTP061 antibody
    • OTTHUMP00000217744 antibody
    • OTTHUMP00000217745 antibody
    • OTTHUMP00000217746 antibody
    • PROM1 antibody
    • PROM1_HUMAN antibody
    • Prominin I antibody
    • Prominin like 1 antibody
    • Prominin like protein 1 precursor antibody
    • Prominin mouse like 1 antibody
    • Prominin-1 antibody
    • Prominin-like protein 1 antibody
    • Prominin1 antibody
    • PROML1 antibody
    • RP41 antibody
    • STGD4 antibody
    see all

Images

  • All lanes : Anti-CD133 antibody [mAbcam27699] (ab27699) at 5 µg/ml

    Lane 1 : Caco-2 whole cell lysate
    Lane 2 : HT-29 whole cell lysate
    Lane 3 : Mouse kidney normal tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-mouse at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 55 kDa (possible IgG)


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab27699 overnight at 4°C. Antibody binding was detected using a goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

References

This product has been referenced in:
  • Nosrati A  et al. Cancer Stem Cell Markers CD44, CD133 in Primary Gastric Adenocarcinoma. Int J Mol Cell Med 3:279-86 (2014). IHC-P ; Human . Read more (PubMed: 25635255) »
  • Lubanska D  et al. The cyclin-like protein Spy1 regulates growth and division characteristics of the CD133+ population in human glioma. Cancer Cell 25:64-76 (2014). ICC/IF ; Human . Read more (PubMed: 24434210) »
See all 5 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Question
Answer

Thank you for your inquiry.

Unfortunately, we do not have any antibodies againstCD133 available in our catalog.
I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:
www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit:

http://www.epitomics.com/services/
email: service@epitomics.com

Alternatively, please contact Li Li at Epitomics:

li.li@epitomics.com

Read More

Answer

Thank you for contacting us.

Does FC mean flow cytometry? According to our records, ab27699 was tested for WB and IHC-P only.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse day 5 colon)
Specification
Mouse day 5 colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 07 2009

Application
Flow Cytometry
Sample
Human Cell (KM-H2)
Specification
KM-H2
Preparation
Cell harvesting/tissue preparation method: Asynchronous KM-H2 were harvested and labeled by indirect immunofluorescence
Sample buffer: PBS + 5% FBS
Permeabilization
No
Gating Strategy
Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive.

Dr. Kirk Mcmanus

Verified customer

Submitted Dec 16 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (jejunum)
Specification
jejunum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Mr. Carl Hobbs

Verified customer

Submitted May 07 2008

Application
Western blot
Sample
Human Cell lysate - whole cell (Multiple cell lines, including U2OS)
Loading amount
25 µg
Specification
Multiple cell lines, including U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Feb 25 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 14 2007

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Renal (proximal tuular) epithelial cell line)
Specification
Renal (proximal tuular) epithelial cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Tritonx-100 (PBS)
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 14 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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