Recombinant Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1168Y] to OX40L/TNFSF4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-OX40L/TNFSF4 antibody [EP1168Y]
See all OX40L/TNFSF4 primary antibodies -
Description
Rabbit monoclonal [EP1168Y] to OX40L/TNFSF4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HUVEC and A-172 whole cell lysate. Recombinant human CD252 / OX40L.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1168Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. -
Involvement in disease
Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7292 Human
- Omim: 603594 Human
- SwissProt: P23510 Human
- Unigene: 181097 Human
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Alternative names
- CD 134L antibody
- CD 252 antibody
- CD134 ligand antibody
see all
Images
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All lanes : Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : A-172 (human brain glioblastoma) whole cell lysate
Lane 3 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 6 secondsOX40L/TNFSF4 is a glycoprotein. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8642328)
Negative control: Raji (PMID: 8642328)
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All lanes : Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130) at 1/50000 dilution
Lane 1 : Recombinant human CD252 / OX40L at 0.01 µg
Lane 2 : Recombinant human CD252 / OX40L at 0.005 µg
Secondary
All lanes : Goat anti-rabbit-HRP at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?The recombinant protein was expressed as a fusion protein.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab76130 has been referenced in 1 publication.
- Wang X et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med 12:3242-3248 (2016). IHC-P ; Rabbit . PubMed: 27882144