FITC Anti-CD3 antibody [UCHT1] (ab34275)
Key features and details
- FITC Mouse monoclonal [UCHT1] to CD3
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD3 antibody [UCHT1]
See all CD3 primary antibodies -
Description
FITC Mouse monoclonal [UCHT1] to CD3 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Tissue/ cell preparation (Human) -infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
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Positive control
- human peripheral blood lymphocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant -
Clonality
Monoclonal -
Clone number
UCHT1 -
Myeloma
P3-NS1/1-Ag4-1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)
- PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)
- PerCP/Cy5.5® Anti-CD3 antibody [UCHT1], prediluted (ab157317)
- Biotin Anti-CD3 antibody [UCHT1] (ab191112)
- APC Anti-CD3 antibody [UCHT1] (ab239287)
- redFluor™ 710 Anti-CD3 antibody [UCHT1] (ab241935)
- violetFluor™ 450 Anti-CD3 antibody [UCHT1] (ab241941)
- PE/Cy7® Anti-CD3 antibody [UCHT1] (ab81992)
- APC/Cy7® Anti-CD3 antibody [UCHT1] (ab82013)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | (5) |
Use at an assay dependent concentration.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Images
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Flow Cytometry analysis of Human peripheral blood lymphocytes staining CD3 with ab34275 at 5µL (1.0µg) concentration (solid line), and isotype control Mouse IgG1 (FITC) at 1.0µg (dotted line).
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ab34275 staining CD3 in Human PBMCs by Flow Cytometry. PBMCs were isolated from buffy coat after Ficoll preparation in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy: Live Lymphocytes. -
FACS analysis of CD3 expression in human peripheral blood lymphocytes stained with ab34275 (solid line) at 0.25 ug and 0.25 ug isotype control (dashed line).
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab34275 has been referenced in 11 publications.
- Dang G et al. T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2. Redox Biol 50:102257 (2022). PubMed: 35149342
- Lan J et al. Cord Blood Natural Killer Cells Inhibit Sepsis Caused by Feces-Induced Acute Peritonitis via Increasing Endothelium Integrity. Cell Transplant 31:9636897221090257 (2022). PubMed: 35438589
- Cui C et al. Propofol maintains Th17/Treg cell balance in elderly patients undergoing lung cancer surgery through GABAA receptor. BMC Immunol 23:58 (2022). PubMed: 36434505
- Di N et al. Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia. Oncol Lett 18:35-42 (2019). PubMed: 31289469
- Garofalo M et al. Extracellular vesicles enhance the targeted delivery of immunogenic oncolytic adenovirus and paclitaxel in immunocompetent mice. J Control Release 294:165-175 (2019). PubMed: 30557650