Recombinant Anti-CD300a antibody [EPR5226] (ab124810)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5226] to CD300a
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD300a antibody [EPR5226]
See all CD300a primary antibodies -
Description
Rabbit monoclonal [EPR5226] to CD300a -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD300a aa 50-150. The exact sequence is proprietary.
-
Positive control
- Human lymph node and spleen lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.90 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5226 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa). |
Target
-
Function
Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. -
Tissue specificity
Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes. -
Sequence similarities
Belongs to the CD300 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPhosphorylated on tyrosine. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 11314 Human
- Omim: 606790 Human
- SwissProt: Q9UGN4 Human
- Unigene: 9688 Human
-
Alternative names
- CD300 antigen-like family member A antibody
- CD300a antibody
- CD300a antigen antibody
see all
Images
-
All lanes : Anti-CD300a antibody [EPR5226] (ab124810) at 1/1000 dilution
Lane 1 : Human lymph node lysates
Lane 2 : Human spleen lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab124810 has not yet been referenced specifically in any publications.