Overview

  • Product name
  • Description
    Rabbit polyclonal to CD4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Rat CD4 aa 420-457 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    RCRHQQRQAARMSQIKRLLSEKKTCQCSHRMQKSHNLI


    Database link: P05540

  • Positive control
    • IHC-P: Human colon carcinoma, rat kidney, human kidney and rat lung tissues. WB: CD4 recombinant protein; Raji cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab203034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 51 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Target

  • Function
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization
    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Images

  • Anti-CD4 antibody (ab203034) at 1/300 dilution + U937 Cell Lysate at 40 µg

    Secondary
    IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling CD4 with ab203034 at 1/200 dilution overnight, followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C.

  • Anti-CD4 antibody (ab203034) at 1/300 dilution + HL60 Cell Lysate at 40 µg

    Secondary
    IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

    Predicted band size: 51 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded rat kidney tissue labeling CD4 with ab203034 at 1/400 dilution overnight at 4°C followed by a conjugated secondary for 20 minutes and DAB staining.

  • All lanes : Anti-CD4 antibody (ab203034) at 1/300 dilution

    Lane 1 : CD4 recombinant protein
    Lane 2 : Raji cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution

    Predicted band size: 51 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling CD4 with ab203034 at 1/400 dilution followed by conjugation to the secondary antibody and DAB staining.

References

This product has been referenced in:
  • Zhang W  et al. Correlation and clinical significance of LC3, CD68+ microglia, CD4+ T lymphocytes, and CD8+ T lymphocytes in gliomas. Clin Neurol Neurosurg 168:167-174 (2018). IHC-P ; Human . Read more (PubMed: 29567578) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Skeletal muscle)
Permeabilization
No
Specification
Skeletal muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Sep 11 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (lymph node and thymus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 4, 6, 9
Permeabilization
No
Specification
lymph node and thymus
Blocking step
Ultratek HRP (anti-polyvalent), ScyTek Laboratories, Logan, Utah, USA as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Sara Belluco

Verified customer

Submitted May 26 2017

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